NECAT Home Page

The Northeastern Collaborative Access Team (NE-CAT) facility at the Advanced Photon Source at Argonne National Laboratory is managed by Cornell University and consists of seven member institutions:

Columbia University

Cornell University

Harvard University

Memorial Sloan-Kettering Cancer Center

Massachusetts Institute of Technology

Rockefeller University

Yale University.

Primary funding for this project comes from the National Center for Research Resources (NCRR), a division of the National Institutes of Health (NIH). Additional financial support for NE-CAT comes from the member institutions.

Sequence-matched MutM Interrogation Complex 5 (IC5)	E. coli 70S ribosome	Urea Transporter from Desulfovibrio Vulgaris	MutY bound to its inhibitor DNA
Nup84-Nup145C-Sec13 Edge Element	Simvastatin Synthase (LovD)	CHK2 DNA-Damage Checkpoint Kinase	Histidine Acid Phosphatase
Rru_A2000 from Rhodospirillum rubrum: A cupin-2 domain	SPOP-Substrate Complexes	Mms21 and Smc5 Complex	P. aeruginonsa RssB
Human SepSecS-tRNASec complex	T. thermophilus Argonaute E	Amicyanin	Inhibitor Bound to Cathepsin S
EF-P Bound to 70S Ribosome	Putative Alcohol Dehydrogenase (TM_O42) from Themotoga maritima	N-terminal Domain of E. coli RssB	Fast Activating G Protein Mutant
Apo CAP	SUMO E3 Ligase SIZ1	Adenosine Deaminase from Plasmodial vivax	Mutant Bacteriorhodopsins
Human Nucleoporins Nup170 and 133	CBC-Importin Alpha Complex	Membrane Fusion Protein CusB	Carboxysome Small Shell Protein CsoS1C
Thermotoga maritima Soluble Receptor (Tm14)	Ran-Binding Zinc Finger Domain	Nucleoporin Nup120	Anaphase Promoting Complex TPR Subdomain Assembly
Acyl-CoA Thioesterase (ACOT2)	Rv3019c-Rv3020c (Mycobacterium tuberculosis)	EPHA2 Ligand-Binding Domain	ATP-gated P2X₄ Ion Channel
(+)-δ-Cadinene Synthase	Islet Amyloid Polypeptide	Human Plasma Platelet Activating Factor Acetylhydrolase	Rotavirus Outer Capsid Protein VP7
GluR2 Amino-Terminal Domain	Human PACSIN2 F-BAR Domain	Human SIRT3	Escherichia coli Enzyme MccB
Cytidine Deaminase	Middle and Carboxy-Terminal Domain of HSP90	Human DNA Polymerase Iota	MogR Repressor
Mus musculus Iodotyrosine Deiodinase	Dpo4 Extension Ternary Complex	Islet Amyloid Segments	Human Ubiquitin-Activating Enzyme 5
Rv0301 Rv0300 Toxin Antitoxin Complex from Mycobacterium tuberculosis	Vascular Protein von Willebrand Factor A2 Domain	> Tomato Aspermy Virus 2b in Complex with siRNA	> JARID1A-PHD3 Complexed with H3(1-9)K4me3 Peptide
Toxin-Antitoxin Complex RelBE2 (RV2865-2866)	Dengue Virus Type 1 Envelope Protein	Ribosome with Antibiotics	RAG1 Nonamer-binding Domain
DegS H198P/D320A mutant	Human NEMO CC2_LZ Domain	Human Mitochondrial Peptide Deformylase	Human Purine Nucleoside Phosphorylase
Putative Oxidoreductase from Bacillus subtilis	Putative OORC Subunit of 2-OxoGlutarate:Acceptor Oxidoreductase	UvrA-UVvrB Interaction Domains	PDZ-Fibronectin Fusion Protein
NE1^ufd-UBE2F^core	Pyridoxal Phosphate Biosynthetic Protein (Burkholderi Pseudomallei)	2-Methyl-3-hydroxypyridine-5-carboxylic acid Oxygenase	Glyceraldehyde-3-Phosphate Dehydrogenase
Polo-Box Domain of Mammalian Polo-Like Kinase 1	Macrolide Biosensor Protein, MphR(A)	RB69 DNA Polymerase Triple Mutant	Glutamine-Dependent NAD⁺ Synthetase
Putative TRNA Synthetase	Proline Dehydrogenase	cAMP Receptor Protein from M. tuberculosis	Ankyrin-Binding Site of β-Spectrin
Geobacillus stearothermophilus KinB	DDB1-DDB2 Complex	Tapasin/ERp57 Heterodimer	FMN Riboswitch Bound to FMN
Mouse VDAC1	Notch1 Negative Regulatory Region	Human Histone Deacetylase 8	Putative Glucosidase lplD from Bacillus subtilis
Bacterial N-end Rule Adapter ClpS	Human H/ACA RNP Assembly Protein shq1	Fcp1, an Essential RNA Polymerase II CTD Phosphatase	Muscleblind-Like MBNL1
Group II Intron	Transcribing t7 RNA Polymerase	Nucleoporin Complex	Human S-adenosylmethionine Decarboxylase
IsocitrateLyase from Brucella melitensis	AtNOS1/AtNOA1 Ortholog	Caulobacter crescentus HMP-P synthase	KEOPS Complex
Shigella VirA	Binary Complex of Citryl Dethia COA and Citrate Synthase	Protein-Translocation Complex (SecY Channel/SecA ATPase)	SMK Box Riboswitch
Endophilin and Sorting Nexin 9	DNA Polymerase III α-Subunit	Large Ribosomal Subunit of Haloarcula Marismortui	CysO/CysM
Putative Methyltransferase-MM_2633 from Methanosarcina mazei	Thermotoga maritima Lysine Riboswitch	DegS-deltaPDZ q191A	Human SENP7 Catalytic Domain
IsocitrateLyase from Brucella melitensis	Guide-Strand-Containing Argonaute Silencing Complex	AlkA Host/Guest Complex	Sulfur Carrying Protein Complex Mycobacterium tuberculosis
Rotavirus SA11 VP1 / RNA Complexes	SAM-dependent methyltransferase from Bacteroides vulgatus ATCC 8482	Quinolinate synthase from Pyrococcus furiosus	Nipah G Attachment Glycoprotein
PurLQS Thermotoga maritima	TRAPP Subassembly Activating the Rab Ypt1p	VapBC-5 from Mycobacterium tuberculosis	Peptidoglycan Glycosyltransferase Domain from Aquifex aeolicus
Putative Transglycosylase from Caulobacter crescentus	Nup107/Nup133	HIV Vif BC-box in Complex with Human ElonginB and ElonginC	NF-κB Inhibitor A20
B-subunit of the DNA gyrase from Myxococcus xanthus	RecA-ssDNA/dsDNA	Anisomycin resistant 50S Ribosomal Subunit	Platinum-DNA Adduct Bound to Deoxyguanosine
Human Factor VIII	PCNA12 Dimer from Sulfolobus solfataricus	Self-spliced Group II Intron	Prosubtilisin Substrate Complex of Subtilisin
Pyruvate Kinase M2	Vitronectin/Urokinase/Urokinase Receptor complex	L7/L12 stalk of 50S Large Ribosomal Subunit	Hepatitis C Virus IRES Subdomain IIa
CNK/HYP Dimer	Thiamin Monophosphate Kinase Aquifex aeolicus	Bacterial Carboxysome Cell	PDCD4 MA3 Domains
Ankyrin Repeat Domain of TRPV6	Oligogalacturonate lyase (VPA0088) from Vibrio Parahaemolyticus	DR_0571 Protein from Deinococcus radiodurans	HTH-type Transcriptional Regulator AcrR
EGFR - Kinase Domain	Thiamin Binding Protein	S. cerevisiae Dcn1	Metal Binding Domain of NikR
Human BPTF PHD Finger	PCNA123 Heterotrimer	Violacein Biosynthetic Enzyme VioE	Bacillus stearothermophilus UvrA
Methanocaldococcus jannaschii PurP	Pyrococcus furiosusPurP	C-Terminal Kinase Domain of P90 2 Ribosomal S6 Kinase 2	DegS, a PDZ Protease
Human Intrinsic factor: Cobalamin complex	DnaB Helicase	HMPD Decarboxylase	Inner Kinetochore Subunit Cep3p
Rebeccamycin Biosynthetic Enzyme RebC	Nucleoporin Nic96	\|Rad4 Nucleotide Excision Repair Protein	AMP-activated Protein Kinase
Pyrrolysyl-tRNA Synthetase	ADH Synthase	Lowe Syndrome Protein OCRL	C. crescentus SspB Ortholog
Yeast Sec13/31 Vertex Element	Yeast Fatty Acid Synthase	Minimal Pluripotent DNA Ligase	Phi29 DNA Polymerase
At1g77540-Coenzyme A Complex	Human Mcl-1 in Complex with Bim BH3	SCF^Cdc4 Dimer	Focal Adhesion Kinase
Non-covalent Complex Between Human SUMO-1 and Human Ubc9	Fbw7-Skp1-Cyclin E Complex	FH2 domain of Daam1	DNA Ligase Bound to Nicked DNA-Adenylate
Herpesvirus-encoded Cysteine Protease	Monobody MBP-74/Maltose Binding Protein Fusion Complex	Lung Cancer-Derived EGFR	Death Domain Containing Protein CRADD
Inosine 5'-monophosphate cyclohydrolase Methanobacterium thermoautotrophicum	Rab GTPase Sec4p	Peptidoglycan Glycosyltransferase	Amicyanin
Q7WAF1 BORPA from Bordetella parapertussis	4-Hydroxybutyrate Coenzyme A Transferase	Q9HYQ7 PSEAE from Pseudomonas aeruginosa	Escherichia coli N⁵-carboxyaminoimidazole Ribonucleotide Mutase
Aromatic Amine Dehydrogenase	Synthetic Human Lysozyme	Eurkaryotic RNA Exosome	Hydroxylase-Regulatory Protein Complex
T4 RNA Ligase 2	TmYaaDE	Thermotoga maritima Formylglycinamide Ribonucleotide Amidotransferase	Human Senp2 in Complex with PreSUMO-2
HTH-type Transcriptional Regulator yddM	FeeM, an N-Acyl Amino Acid Synthase	RNAase II	Arabidopsis thaliana Double Bond Reductase (at5G16970)
DNA polymerase III alpha subunit Thermus aquaticus	Glycoprotein B from Herpes Simplex virus 1	P52G Mutant of Amicyanin	Self-inhibited Thrombin
Active Chimera of (S)-Mandelate Dehydrogenase	Ankyrin Repeat Domain - TRPV2 Ion Channel	BMP2-induced 3-kb Gene Protein	tRNA Splicing Endonuclease
Mouse Pyrimidine 5'-Nucleotidase Type 1	Arabidopsis thaliana At1g77540	DNA Glycosylase	BPTF PHD Finger of NURF
Cbf5-Nop10-Gar1 Complex	Rb C-terminal Domain bound to E2F1-DP1	5'-Deoxy-5'-Methylthioadenosine Phosphorylase II	Ubiquitin-Conjugating Enzyme E2
RNAase A	Exocyst Subunit Exo70p	Escherichia coli ThiFS	Arsenical Resistance Protein
Human Deoxycytidine Kinase	Type IIA DNA Topoisomerase	Fe(II) HppE	Skp1-Skp2-Cks1-p27^Kip1 Complex
pH Domain of SKAP55	Eschrichia coli Uridine Phosphorylase	Succinylglutamate Desuccinylase Escherichia coli	Toxoplasma gondii Adenosine Kinase
3-Hydroxyanthranilate-3,4-dioxygenase	SWI2/SNF2 Chromatin-Remodeling Domain	14-3-3	Sspb Heterodimer
Ubiquitin Hydrolase UCH-L3	Bacillus subtilis TenI	Bacillus subtilis TenA	Trichomonas vaginalis Purine Nucleoside Phosphorylase
Hypothetical Protein (Tm1631) Thermotoga Maritima	Permutated Arc Repressor	Sspb-Rsea Complex	Human Tub Protein (Isoform A)
Protein-primed DNA Polymerase of Bacteriophage Phi29	Bacterial Nucleoside Transporter Tsx	Sspb Disulfide	Hypothetical Protein Vc1974 from Vibrio Cholerae
Cytochrome c and Cytochrome c Peroxidase	Achaeoglobus fulgidus tRNA Nucleotidyltransferase	Ytnj from Bacillus subtilis	Phage Twort Group I Ribozyme Product Complex
Long-Chain Fatty Acid Transporter FadL	Thiazole Synthase/Sulfur Carrier Protein Complex from Bacillus subtilis	Aminoimidazole Riboside Kinase from Salmonella enterica	Lysine5,6-aminomutase
T. maritima S-adenosylmethionine Decarboxylase	Bni1p Formin Homology-2 domain	a/bSerine Hydrolase (Ydr428C)	B. subtilis PurS
Protein Conducting Channel	Purine 2'-deoxyribosyltransferase	E. coli AMP Nucleosidase	Nogo-66 Receptor (NgR)
Nickel-Responsive Transcription Factor NikR	Semaphorin-3A Receptor binding Module	Human phosphopantothenoylcysteine (PPC) Decarboxylase	YaaE
Pvl ArgDC	ThiO	Human Phosphopantothenoylcysteine Synthetase	Large ribosomal E site Complexes

Recent Highlights

10/07/2009

*NE-CAT's executive committee member Thomas Steitz shares 2009 Nobel Prize in Chemistry*

The Nobel Assembly at the Karolinska Institute in Sweden announced today that Thomas Steitz is one of three winners of the 2009 Nobel Prize in Chemistry for his work describing the structure and function of the ribosome. Thomas Steitz is a member of NE-CAT's executive committee and a frequent user of NE-CAT beamlines.

09/03/2009

Crystal Structure of the ATP-Gated P2X4 Ion Channel in the Closed State, Toshimitsu Kawate, Jennifer Carlisle Michel, William T. Birdsong, and Eric Gouaux, Nature 460, 592-598 (2009)

Formation of the First Peptide Bond: the Structure of EF-P Bound to the 70S Ribosome, Gregor Blaha, Robin E. Stanley, and Thomas A. Steitz, Science 325, 966-970 (2009)

Structures of the Ribosome in the Intermediate States of Ratcheting, Wen Zhang, Jack A. Dunkle and Jamie H.D. Cate, Science 325, 1014-1017 (2009)

09/02/2009

August 2009 status report now available.

08/06/2009

July 2009 status report now available.

06/30/2009

June 2009 status report now available.

06/18/2009

Looking for Ways to Improve Vaccines Against the Deadly Rotavirus

06/09/2009

May 2009 status report now available.

05/01/2009

April 2009 status report now available.

03/06/2009

February 2009 status report now available.

02/16/2009

New MD2 installed on 24-ID-C beamline with sample placement robot now available to users.

02/09/2009

January 2009 status report now available.

1/23/2009

NE-CAT Resource Advisory Committee Meets. See pic.

1/13/2009

December 2008 Status report available.

12/11/2008

November 2008 Status report available.

11/04/2008

Featured Publication

Structure of a complex of the ATPase Sec A and the protein-translocation channel J. Zimmer, Y. Nam & T. Rapoport

Nature 455, 936-945 (2008)

Click here for full article.

11/04/2008

NE-CAT’s second MD2 microdiffractometer is delivered.