NECAT Beamline

The Northeastern Collaborative Access Team (NE-CAT) facility at the Advanced Photon Source at Argonne National Laboratory is managed by Cornell University and consists of seven member institutions:

  • Columbia University
  • Cornell University
  • Harvard University
  • Memorial Sloan-Kettering Cancer Center
  • Massachusetts Institute of Technology
  • Rockefeller University
  • Yale University.

    • Primary funding for this project comes from the National Center for Research Resources (NCRR), a division of the National Institutes of Health (NIH). Additional financial support for NE-CAT comes from the member institutions.

    2009 Staff Activities

    Publications

    Singh, H., Felts, R. L., Schuermann, J. P., Reilly, T. J., and Tanner, J. J. (2009) Crystal Structures of the Histidine Acid Phosphatase from Francisella tularensis Provide Insight into Substrate Recognition, J. Mol. Biol. 394, 893-904. PubMed

    Schmeing, T. M., Voorhees, R. M., Kelley, A. C., Gao, Y. G., Murphy, F. V. t., Weir, J. R., and Ramakrishnan, V. (2009) The Crystal Structure of the Ribosome Bound to EF-Tu and Aminoacyl-tRNA, Science 326, 688-694. PubMed

    Galaleldeen, A., Strange, R., Whitson, L. J., Antonyuk, S., Narayana, N., Taylor, A. B., Schuermann, J. P., Holloway, S. P., Hasnain, S. S., and Hart, P. J. (2009) Structural and Biophysical Properties of Metal-Free Pathogenic SOD1 Mutants A4V and G93A, Arch Biochem. Biophys. (in press) PubMed

    Wiltzius, J. J., Landau, M., Nelson, R., Sawaya, M. R., Apostol, M. I., Goldschmidt, L., Soriaga, A. B., Cascio, D., Rajashankar, K., and Eisenberg, D. (2009) Molecular mechanisms for protein-encoded inheritance, Nat. Struct. Mol. Biol. 16, 973-978. PubMed

    Wang, J., Dye, B. T., Rajashankar, K. R., Kurinov, I., and Schulman, B. A. (2009) Insights into anaphase promoting complex TPR subdomain assembly from a CDC26-APC6 structure, Nat. Struct. Mol. Biol. 16, 987-989. PubMed

    Choi, M., Sukumar, N., Liu, A., and Davidson, V. L. (2009) Defining the Role of the Axial Ligand of the Type 1 Copper Site in Amicyanin by Replacement of Methionine with Leucine, Biochemistry 48, 9174-9184.PubMed

    Neau, D. B., Gilbert, N. C., Bartlett, S. G., Boeglin, W., Brash, A. R., and Newcomer, M. E. (2009) The 1.85 Å Structure of an 8R-Lipoxygenase Suggests a General Model for Lipoxygenase Product Specificity, Biochemistry 48, 7906-7915. PubMed

    Su, C.-C., Yang, F., Long, F., Reyon, D., Routh, M. D., Kuo, D. W., Mokhtari, A. K., Van Ornam, J. D., Rabe, K. L., Hoy, J. A., Lee, Y. J., Rajashankar, K. R., and Yu, E. W. Crystal structure of the membrane fusion protein CusB from Escherichia coli, J. Mol. Biol. 393, 342-355. PubMed

    Schuette, J. C., Murphy, F. V. IV, Kelley, A. C., Weir, J. R., Giesebrecht, J., Connell, S. R., Loerke, J., Mielke, T., Zhang, W., Penczek, P. A., Ramakrishnan, V., and Spahn, C. M. (2009) GTPase activation of elongation factor EF-Tu by the ribosome during decoding, EMBO J. 28, 755-765. PubMed

    Wang, Q., Navarro, M. V., Peng, G., Molinelli, E., Shih, L. G., Judson, B. L., Rajashankar, K. R., and Sondermann, H. (2009) Molecular mechanism of membrane constriction and tubulation mediated by the F-BAR protein Pacsin/Syndapin, Proc. Natl. Acad. Sci. U. S. A. 106, 12700-12705. PubMed

    Sukumar, N., Mathews, F. S., Gordon, M. M., Ealick, S. E., and Alpers, D. H. (2009) Postcrystallization Analysis of the Irreproducibility of the Human Intrinsic Factor-Cobalamin Complex Crystals, Cryst. Growth Des. 9, 348-351. PubMed

    Himanen, J. P., Goldgur, Y., Miao, H., Myshkin, E., Guo, H., Buck, M., Nguyen, M., Rajashankar, K. R., Wang, B., and Nikolov, D. B. (2009) Ligand recognition by A-class Eph receptors: crystal structures of the EphA2 ligand-binding domain and the EphA2/ephrin-A1 complex, EMBO Rep. 10, 722-728. PubMed

    Sukumar, N., Dewanti, A., Merli, A., Rossi, G. L., Mitra, B., and Mathews, F. S. (2009) Structures of the G81A mutant form of the active chimera of (S)-mandelate dehydrogenase and its complex with two of its substrates, Acta Crystallogr. D 65, 543-552. PubMed

    Bick, M. J., Lamour, V., Rajashankar, K. R., Gordiyenko, Y., Robinson, C. V., and Darst, S. A. (2009) How to Switch Off a Histidine Kinase: Crystal Structure of Geobacillus stearothermophilus KinB with the inhibitor Sda, J. Mol. Biol. 386, 163-177. PubMed

    Ostrander, E. L., Larson, J. D., Schuermann, J. P., and Tanner, J. J. (2009) A Conserved Active Site Tyrosine Residue of Proline Dehydrogenase Helps Enforce the Preference for Proline over Hydroxyproline as the Substrate Biochemistry 48, 951-959. PubMed

    Presentations

    Use of Micro-focused Beamline in Solving the Crystal Structure of the Avian Thymic Hormone (ATH) in Calcium Bound Form, Jon Schuermann, 2009 ACA Meeting, Toronto CA, July 25-30, 2009.

    Malcolm Capel, “Recent Operating and Development Experiences with the NE-CAT ACCEL/Maatel MD2 Microdiffractometer”, APS Technical Working Group (TWG) Meeting, April 16, 2009.

     

    Poster Sessions

    Microdiffraction-What Can be Done?, K. Rajashankar, M. Capel, S. Harrison, and S. Ealick, 2009 ACA Meeting, Toronto CA, July 25-30, 2009

    Northeastern Collaborative Access Team ( NE- CAT) Beamlines at the Advanced Photon Source, I. Kourinov, S. Ealick, M. Capel, F. Murphy,   D. Neau, K. Perry, K. Rajashankar, J. Schuermann, and N. Sukumar, 2009 ACA Meeting, Toronto CA, July 25-30, 2009

     

    Committee Memberships

    K. Rajashankar, APS Beamtime Allocation Committee

    M. Capel, BioCAT Resource Advisory Committee

    M. Capel, Partner User Council (PUG)

    Malcolm Capel, Design Committee of the Proteins to Organisms Imaging Beamline, Renewal of the Advanced Photon Source Project

     

    2008 Staff Activities

    Publications

    Ostrander, E. L., Larson, J. D., Schuermann, J. P., and Tanner, J. J. (2009) A Conserved Active Site Tyrosine Residue of Proline Dehydrogenase Helps Enforce the Preference for Proline over Hydroxyproline as the Substrate Biochemistry 48, 951-959. PubMed

    Sukumar, N., Mathews, F. S., Gordon, M. M., Ealick, S. E., and Alpers, D. H. (2008) Postcrystallization Analysis of the Irreproducibility of the Human Intrinsic Factor-Cobalamin Complex Crystals, Cryst. Growth Des. 9, 348-351. URL

    Brohawn, S. G., Leksa, N. C., Spear, E. D., Rajashankar, K. R., and Schwartz, T. U. (2008) Structural Evidence for Common Ancestry of the Nuclear Pore Complex and Vesicle Coats, Science 322, 1369-1373 PubMed

    Toor, N., Rajashankar, K., Keating, K. S., and Pyle, A. M. (2008) Structural basis for exon recognition by a group II intron, Nat. Struct. Mol. Biol. 15, 1221-1222. PubMed

    Mao, D. Y., Neculai, D., Downey, M., Orlicky, S., Haffani, Y. Z., Ceccarelli, D. F., Ho, J. S., Szilard, R. K., Zhang, W., Ho, C. S., Wan, L., Fares, C., Rumpel, S., Kurinov, I., Arrowsmith, C. H., Durocher, D., and Sicheri, F. (2008) Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine, Mol. Cell 32, 259-275. PubMed

    Xu, K., Rajashankar, K. R., Chan, Y. P., Himanen, J. P., Broder, C. C., and Nikolov, D. B. (2008) Host cell recognition by the henipaviruses: crystal structures of the Nipah G attachment glycoprotein and its complex with ephrin-B3, Proc. Natl. Acad. Sci. U. S. A. 105, 9953-9958. PubMed

    Rajakulendran, T., Sahmi, M., Kourinov, I., Tyers, M., Therrien, M., and Sicheri, F. (2008) CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling, Proc. Natl. Acad. Sci. U. S. A. 105, 2836-2841. PubMed

    Soriano, E. V., Rajashankar, K. R., Hanes, J. W., Bale, S., Begley, T. P., and Ealick, S. E. (2008) Structural Similarities between Thiamin-Binding Protein and Thiaminase-I Suggest a Common Ancestor, Biochemistry 47, 1346-1357. PubMed

    Lin, S. C., Chung, J. Y., Lamothe, B., Rajashankar, K., Lu, M., Lo, Y. C., Lam, A. Y., Darnay, B. G., and Wu, H. (2008) Molecular Basis for the Unique Deubiquitinating Activity of the NF- kB Inhibitor A20, J. Mol. Biol. 376, 526-540. PubMed

    Hlinkova, V., Xing, G., Bauer, J., Shin, Y. J., Dionne, I., Rajashankar, K. R., Bell, S. D., and Ling, H. (2008) Structures of monomeric, dimeric and trimeric PCNA: PCNA-ring assembly and opening, Acta Crystallogr. D 64, 941-949. PubMed

    Weixlbaumer and F. Murphy”Crystallographic Studies of Decoding by Modified Bases: Correlation of Structure and Function”, in “ DNA and RNA Modification Enzmes: Comparative Structure, Mechanism, Functions, Cellular Interactions and Evolution:. Editor Henri Grosjean, To be published Landes Bioscience, 2009.

     

    Presentations

    Dealing With Non-Merohedral Twinning, K, Rajashanker, ACA 2008, Knoxville, TN, May 31-June5

    SAD Data Collection and Experimental Setup, K. Rajashankar, 66 th Annual Pittsburg Diffraction Conference, October 30, 2008

    Crystallographic Studies on a Vitamin B12 Transport Protein and an Electronic Transfer Complex, N. Sukumar ANL Biosciences Division Seminar, September 25, 2008

     

    Poster Presentations

    K. Rajashankar, Microdiffraction: What Can Be Done? APS User Week 2007

    K. Rajashankar, Microdiffraction: What Can Be Done? NCRR Biomedical Technology Centers Principal Investigator Meeting, Bethesda 2008

    I. Kourinov et al, Implementation of Microdiffraction Techniques at Northeastern CAT Beamlines, International Union of Crystallography, Osaka, Japan 2008

    F. Murphy, “Alloy- Data Processing for Multi-Crystal Macromolecular Crystallography, APS User Week 2008

     

    Workshops Organized

    Engage Your Brain, K. Rajashankar and Z. Dauter, ACA 2008, Knoxville, TN May31-June5

    Software for Challenging Cases in Macromolecular Crystallography, I. Kourinov and F. Murphy, APS User Week May 2008

     

    Memberships and Awards

    K. Rajashankar, APS Beamtime Allocation Committee

    J. Unik, Outstanding Alumnus 2008, Biological, Chemical, and Physics Sciences Department, Illinois Institute of Technology

     
     

    © 2005 Northeastern Collaborative Access Team

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