NECAT Beamline

The Northeastern Collaborative Access Team (NE-CAT) facility at the Advanced Photon Source at Argonne National Laboratory is managed by Cornell University and consists of seven member institutions:

  • Columbia University
  • Cornell University
  • Harvard University
  • Memorial Sloan-Kettering Cancer Center
  • Massachusetts Institute of Technology
  • Rockefeller University
  • Yale University.
  •  

    Primary funding for this project comes from the National Institute of General Medical Sciences(NIGMS) , a division of the National Institutes of Health (NIH). Additional financial support for NE-CAT comes from the member institutions.

    NIGMS Fast Facts

    Supported by:

    National Institutes of Health
    National Institue of General Medical Sciences

    2014 Staff Activities

    Publications

    Xu, Y., Tao, Y., Cheung, L. S., Fan, C., Chen, L.-Q., Xu, S., Perry, K., Frommer, W. B., and Feng, L. (2014) Structures of bacterial homologues of SWEET transporters in two distinct conformations, Nature [Epub ahead of print]. PubMed Also featured in Argonne today.

    Sanches, M., Duffy, N. M., Talukdar, M., Thevakumaran, N., Chiovitti, D., Canny, M. D., Lee, K., Kurinov, I., Uehling, D., Al-Awar, R., Poda, G., Prakesch, M., Wilson, B., Tam, V., Schweitzer, C., Toro, A., Lucas, J. L., Vuga, D., Lehmann, L., Durocher, D., Zeng, Q., Patterson, J. B., and Sicheri, F. (2014) Structure and mechanism of action of the hydroxy-aryl-aldehyde class of IRE1 endoribonuclease inhibitors, Nat. Commun. 5, 4202. PubMed

    Montemayor, E. J., Katolik, A., Clark, N. E., Taylor, A. B., Schuermann, J. P., Combs, D. J., Johnsson, R., Holloway, S. P., Stevens, S. W., Damha, M. J., and Hart, P. J. (2014) Structural basis of lariat RNA recognition by the intron debranching enzyme Dbr1, Nucleic Acids Res. [Epub ahead of print]. PubMed

    Althoff, T., Hibbs, R. E., Banerjee, S., and Gouaux, E. (2014) X-ray structures of GluCl in apo states reveal a gating mechanism of Cys-loop receptors, Nature 512, 333-337. PubMed

    Huo, Y., Nam, K. H., Ding, F., Lee, H., Wu, L., Xiao, Y., Farchione, M. D., Jr., Zhou, S., Rajashankar, K., Kurinov, I., Zhang, R., and Ke, A. (2014) Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation, Nat. Struct. Mol. Biol. 21, 771-777. PubMed

    Yang, G., Fu, Y., Malakhova, M., Kurinov, I., Zhu, F., Yao, K., Li, H., Chen, H., Li, W., Lim, D. Y., Sheng, Y., Bode, A. M., Dong, Z., and Dong, Z. (2014) Caffeic acid directly targets ERK1/2 to attenuate solar UV-induced skin carcinogenesis, Cancer Prev. Res. (Phila) [Epub ahead of print]. PubMed

    Zeqiraj, E., Tang, X., Hunter, R. W., Garcia-Rocha, M., Judd, A., Deak, M., von Wilamowitz-Moellendorff, A., Kurinov, I., Guinovart, J. J., Tyers, M., Sakamoto, K., and Sicheri, F. (2014) Structural basis for the recruitment of glycogen synthase by glycogenin, Proc. Nat. Acad. Sci. U.S.A. 111, E2831-E2840. PubMed

    Lee, K., Zhong, X., Gu, S., Kruel, A. M., Dorner, M. B., Perry, K., Rummel, A., Dong, M., and Jin, R. (2014) Molecular basis for disruption of E-cadherin adhesion by botulinum neurotoxin A complex, Science 344, 1405-1410. PubMed

    Bhattacharya, S., Lou, X., Hwang, P., Rajashankar, K. R., Wang, X., Gustafsson, J. A., Fletterick, R. J., Jacobson, R. H., and Webb, P. (2014) Structural and functional insight into TAF1-TAF7, a subcomplex of transcription factor II D, Proc. Nat. Acad. Sci. U.S.A. 111, 9103-9108. PubMed

    Bolla, J. R., Su, C. C., Do, S. V., Radhakrishnan, A., Kumar, N., Long, F., Chou, T. H., Delmar, J. A., Lei, H. T., Rajashankar, K. R., Shafer, W. M., and Yu, E. W. (2014) Crystal Structure of the Neisseria gonorrhoeae MtrD Inner Membrane Multidrug Efflux Pump, PLoS One 9, e97903. PubMed

    Lei, H. T., Chou, T. H., Su, C. C., Bolla, J. R., Kumar, N., Radhakrishnan, A., Long, F., Delmar, J. A., Do, S. V., Rajashankar, K. R., Shafer, W. M., and Yu, E. W. (2014) Crystal Structure of the Open State of the Neisseria gonorrhoeae MtrE Outer Membrane Channel, PLoS One 9, e97475. PubMed

    Sciara, G., Clarke, O. B., Tomasek, D., Kloss, B., Tabuso, S., Byfield, R., Cohn, R., Banerjee, S., Rajashankar, K. R., Slavkovic, V., Graziano, J. H., Shapiro, L., and Mancia, F. (2014) Structural basis for catalysis in a CDP-alcohol phosphotransferase, Nat. Commun. 5, 4068. PubMed

    Xu, K., Wu, Z., Renier, N., Antipenko, A., Tzvetkova-Robev, D., Xu, Y., Minchenko, M., Nardi-Dei, V., Rajashankar, K. R., Himanen, J., Tessier-Lavigne, M., and Nikolov, D. B. (2014) Structures of netrin-1 bound to two receptors provide insight into its axon guidance mechanism, Science 344, 1275-1279. PubMed

    Demirci, H., Murphy, F. V. t., Murphy, E. L., Connetti, J. L., Dahlberg, A. E., Jogl, G., and Gregory, S. T. (2014) A structural analysis of base substitutions in Thermus thermophilus 16S ribosomal RNA conferring streptomycin resistance, Antimicrob. Agents Chemother. 58, 4308-4317. PubMed

    Su, C. C., Radhakrishnan, A., Kumar, N., Long, F., Bolla, J. R., Lei, H. T., Delmar, J. A., Do, S. V., Chou, T. H., Rajashankar, K. R., Zhang, Q., and Yu, E. W. (2014) Crystal structure of the Campylobacter jejuni CmeC outer membrane channel, Protein Sci. 23, 954-961. PubMed

    Radhakrishnan, A., Kumar, N., Wright, C. C., Chou, T. H., Tringides, M. L., Bolla, J. R., Lei, H. T., Rajashankar, K., Su, C. C., Purdy, G. E., and Yu, E. W. (2014) Crystal structure of the transcriptional regulator Rv0678 of Mycobacterium tuberculosis, J. Biol. Chem. 289, 16526-16540. PubMed

    Dow, B. A., Sukumar, N., Matos, J. O., Choi, M., Schulte, A., Tatulian, S. A., and Davidson, V. L. (2014) The sole tryptophan of amicyanin enhances its thermal stability but does not influence the electronic properties of the type 1 copper site, Arch. Biochem. Biophys. 550-551, 24-27. PubMed

    Jain, R., Rajashankar, K. R., Buku, A., Johnson, R. E., Prakash, L., Prakash, S., and Aggarwal, A. K. (2014) Crystal Structure of Yeast DNA Polymerase & Catalytic Domain, PLoS ONE 9, e94835. PubMed

    Lee, K., Lam, K.-H., Kruel, A. M., Perry, K., Rummel, A., and Jin, R. (2014) High-resolution crystal structure of HA33 of botulinum neurotoxin type B progenitor toxin complex, Biochem. Biophys. Res. Commun. 446, 568-573. PubMed

    Poor, C. B., Wegner, S. V., Li, H., Dlouhy, A. C., Schuermann, J. P., Sanishvili, R., Hinshaw, J. R., Riggs-Gelasco, P. J., Outten, C. E., and He, C. (2014) Molecular mechanism and structure of the Saccharomyces cerevisiae iron regulator Aft2, Proc. Nat. Acad. Sci. U.S.A. 111, 4043-4048. PubMed

    Rajashankar, K., and Dauter, Z. (2014) Data collection for crystallographic structure determination, Methods Mol. Biol. 1140, 211-237. PubMed

    Kobe, M. J., Neau, D. B., Mitchell, C. E., Bartlett, S. G., and Newcomer, M. E. (2014) The Structure of Human 15-Lipoxygenase-2 with a Substrate Mimic, J. Biol. Chem. 289, 8562–8569. PubMed

    Strunk, R. J., Piemonte, K. M., Petersen, N. M., Koutsioulis, D., Bouriotis, V., Perry, K., and Cole, K. E. (2014) Structure determination of BA0150, a putative polysaccharide deacetylase from Bacillus anthracis, Acta Crystallogr. F 70, 156-159. PubMed

    Luo, Z., Rajashankar, K., and Dauter, Z. (2014) Weak data do not make a free lunch, only a cheap meal, Acta Crystallogr. D 70, 253-260. PubMed

    Kumar, N., Radhakrishnan, A., Wright, C. C., Chou, T. H., Lei, H. T., Bolla, J. R., Tringides, M. L., Rajashankar, K. R., Su, C. C., Purdy, G. E., and Yu, E. W. (2014) Crystal Structure of the transcriptional regulator Rv1219c of Mycobacterium tuberculosis, Protein Sci. 23, 423-432. PubMed

    Xu, S., Hermanson, D. J., Banerjee, S., Ghebreselasie, K., Clayton, G. M., Garavito, R. M., and Marnett, L. J. (2014) Oxicams Bind in a Novel Mode to the Cyclooxygenase Active Site via a Two-water-mediated H-bonding Network, J. Biol. Chem. 289, 6799-6808. PubMed

    Huang, H., Zeqiraj, E., Dong, B., Jha, B. K., Duffy, N. M., Orlicky, S., Thevakumaran, N., Talukdar, M., Pillon, M. C., Ceccarelli, D. F., Wan, L. C., Juang, Y. C., Mao, D. Y., Gaughan, C., Brinton, M. A., Perelygin, A. A., Kourinov, I., Guarne, A., Silverman, R. H., and Sicheri, F. (2014) Dimeric Structure of Pseudokinase RNase L Bound to 2-5A Reveals a Basis for Interferon-Induced Antiviral Activity, Mol. Cell 53, 221-234. PubMed

    Presentations

    Frank Murphy, "Synchrotron Beamlines - It's Not Uphill Both Ways Anymore", Cambridge University, July 2014

    Jon Schuermann, "Optimizing Data Collection with micro-diffraction tools", QuoVadis Structural Biology 2014?", June 5-6, 2014, Harvard Medical School.

    Raj Rajashankar,"Dose-sliced data collection", QuoVadis Structural Biology 2014?", June 5-6, 2014, Harvard Medical School.

    David Neau, "Getting the most from remote data collection", QuoVadis Structural Biology 2014?", June 5-6, 2014, Harvard Medical School.

    Jon Schuermann, "RAPD", QuoVadis Structural Biology2014?", June 5-6, 2014, Harvard Medical School.

    Kay Perry, "Automated Merging of Multiple Partial Datasets", 2014 Annual Meeting of the American Crystallographic Association, Albuquerque, NM,, May  20 – May 24, 2014.

    K. R Rajashankar, "Better data, not crystals: Recent advances in Synchrotron MX data collection", The Molecular Biophysics Unit, the Indian Institute of Science, Bangalore, India, February 20, 2014.

    Session Chair

    Jon Schuermann, "Introduction to Data Processing", Quo Vadis Structural Biology 2014? SBGrid/NE-CAT Computing School, Harvard Medical School, Boston, Massachusetts, June 5-7, 2014.

    Posters

    Narayanasami Sukumar. "A comparative analysis on X-ray structure of cobalamin binding proteins", 23rd International Union of Crystallography (IuCr) and General Assembly, August 5–12, 2014, Montreal, Quebec, Canada.

    I. Kourinov, M. Capel, S. Banerjee, F. Murphy, D. Neau, K. Perry, K. Rajashankar, J. Schuermann, N. Sukumar, S. Ealick, "NE-CAT Crystallography Beamlines for Challenging Structural Biology Research", 23rd International Union of Crystallography (IuCr) and General Assembly, August 5–12, 2014, Montreal, Quebec, Canada.

    2013 Staff Activities

    Publications

    Schormann, N., Banerjee, S., Ricciardi, R., and Chattopadhyay, D. (2013) Structure of the uracil complex of Vaccinia virus uracil DNA glycosylase, Acta Crystallogr. F, 1328-1334. PubMed

    Demirci, H., Wang, L., Murphy, F. V. t., Murphy, E. L., Carr, J. F., Blanchard, S. C., Jogl, G., Dahlberg, A. E., and Gregory, S. T. (2013) The central role of protein S12 in organizing the structure of the decoding site of the ribosome, RNA 19, 1791-1801. PubMed

    Marcia, M., Humphris-Narayanan, E., Keating, K. S., Somarowthu, S., Rajashankar, K., and Pyle, A. M. (2013) Solving nucleic acid structures by molecular replacement: examples from group II intron studies, Acta Crystallogr. D 69, 2174-2185. PubMed

    Lee, K., Gu, S., Jin, L., Le, T. T. N., Cheng, L. W., Strotmeier, J., Kruel, A. M., Yao, G., Perry, K., Rummel, A., and Jin, R. (2013) Structure of a Bimodular Botulinum Neurotoxin Complex Provides Insights into Its Oral Toxicity, PLoS Pathog. 9, e1003690. PubMed

    Windsor, M. A., Valk, P. L., Xu, S., Banerjee, S., and Marnett, L. J. (2013) Exploring the molecular determinants of substrate-selective inhibition of cyclooxygenase-2 by lumiracoxib, Bioorg. Med. Chem. Lett. 23, 5860–5864. PubMed

    Shanmugam, G., Minko, I. G., Banerjee, S., Christov, P. P., Kozekov, I. D., Rizzo, C. J., Lloyd, R. S., Egli, M., and Stone, M. P. (2013) Ring-Opening of the γ-OH-PdG Adduct Promotes Error-Free Bypass by the Sulfolobus solfataricus DNA Polymerase Dpo4, Chem. Res. Toxicol. 26, 1348-1360. PubMed

    Kamadurai, H. B., Qiu, Y., Deng, A., Harrison, J. S., Macdonald, C., Actis, M., Rodrigues, P., Miller, D. J., Souphron, J., Lewis, S. M., Kurinov, I., Fujii, N., Hammel, M., Piper, R., Kuhlman, B., and Schulman, B. A. (2013) Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3, Elife 2, e00828. PubMed

    Chen, Y., Rajashankar, K. R., Yang, Y., Agnihothram, S. S., Liu, C., Lin, Y. L., Baric, R. S., and Li, F. (2013) Crystal structure of the receptor-binding domain from newly emerged Middle East respiratory syndrome coronavirus, J. Virol. 87, 10777-10783. PubMed

    Xu, K., Xu, Y., Rajashankar, Kanagalaghatta R., Robev, D., and Nikolov, Dimitar B. (2013) Crystal Structures of Lgr4 and Its Complex with R-Spondin1, Structure 21, 1683–1689. PubMed

    Rutherford, K., Yuan, P., Perry, K., Sharp, R., and Van Duyne, G. D. (2013) Attachment site recognition and regulation of directionality by the serine integrases, Nucleic Acids Res. 41, 8341-8356. PubMed

    Apostol, M. I., Perry, K., and Surewicz, W. K. (2013) Crystal structure of human prion protein fragment reveals a motif for oligomer formation, J. Am. Chem. Soc. 135, 10202−10205. PubMed

    Cantara, W. A., Murphy, F. V. t., Demirci, H., and Agris, P. F. (2013) Expanded use of sense codons is regulated by modified cytidines in tRNA, Proc. Natl. Acad. Sci. U. S. A. 110, 10964–10969. PubMed

    Galaleldeen, A., Taylor, A. B., Chen, D., Schuermann, J. P., Holloway, S. P., Hou, S., Gong, S., Zhong, G., and Hart, P. J. (2013) Structure of the Chlamydia trachomatis Immunodominant Antigen Pgp3, J. Biol. Chem. 288, 22068–22079. PubMed

    Duda, D. M., Olszewski, J. L., Schuermann, J. P., Kurinov, I., Miller, D. J., Nourse, A., Alpi, A. F., and Schulman, B. A. (2013) Structure of HHARI, a RING-IBR-RING Ubiquitin Ligase: Autoinhibition of an Ariadne-Family E3 and Insights into Ligation Mechanism, Structure 21, 1030-1041. PubMed

    Wan, L. C., Mao, D. Y., Neculai, D., Strecker, J., Chiovitti, D., Kurinov, I., Poda, G., Thevakumaran, N., Yuan, F., Szilard, R. K., Lissina, E., Nislow, C., Caudy, A. A., Durocher, D., and Sicheri, F. (2013) Reconstitution and characterization of eukaryotic N6-threonylcarbamoylation of tRNA using a minimal enzyme system, Nucleic Acids Res. 41, 6332-6346. PubMed

    Yu, X., Seegar, T. C., Dalton, A. C., Tzvetkova-Robev, D., Goldgur, Y., Rajashankar, K. R., Nikolov, D. B., and Barton, W. A. (2013) Structural basis for angiopoietin-1-mediated signaling initiation, Proc. Natl. Acad. Sci. U. S. A. 110, 7205-7210. PubMed

    Sartmatova, D., Nash, T., Schormann, N., Nuth, M., Ricciardi, R., Banerjee, S., and Chattopadhyay, D. (2013) Crystallization and preliminary X-ray diffraction analysis of three recombinant mutants of Vaccinia virus uracil DNA glycosylase, Acta Crystallogr. F 69, 295-301. PubMed

    Broussard, T. C., Kobe, M. J., Pakhomova, S., Neau, D. B., Price, A. E., Champion, T. S., and Waldrop, G. L. (2013) The Three-Dimensional Structure of the Biotin Carboxylase-Biotin Carboxyl Carrier Protein Complex of E. coli Acetyl-CoA Carboxylase, Structure 21, 650-657. PubMed

    Sukumar, N. (2013) Crystallographic studies on B12 binding proteins in eukaryotes and prokaryotes, Biochimie 95, 976-988. PubMed

    Demirci, H., Murphy IV, F., Murphy, E., Gregory, S. T., Dahlberg, A. E., and Jogl, G. (2013) A structural basis for streptomycin-induced misreading of the genetic code, Nat. Commun. 4, 1355. PubMed

    Ketkar, A., Zafar, M. K., Maddukuri, L., Yamanaka, K., Banerjee, S., Egli, M., Choi, J. Y., Lloyd, R. S., and Eoff, R. L. (2013) Leukotriene Biosynthesis Inhibitor MK886 Impedes DNA Polymerase Activity, Chem. Res. Toxicol. 26, 221-232. PubMed

    Chae, P. S., Rana, R. R., Gotfryd, K., Rasmussen, S. G., Kruse, A. C., Cho, K. H., Capaldi, S., Carlsson, E., Kobilka, B., Loland, C. J., Gether, U., Banerjee, S., Byrne, B., Lee, J. K., and Gellman, S. H. (2013) Glucose-Neopentyl Glycol (GNG) amphiphiles for membrane protein study, Chem. Commun. (Camb.) 49, 2261-2372. PubMed

    Presentations

    Kanagalaghatta Rajashankar, "Dose-sliced Data Collection", 11th International Conference on Biology and Synchrotron Radiation in Hamburg, Germany, Sept 8-11, 2013.

    Malcolm Capel, "Shutterless Vector Scanned Data Collection Methods for the Pilatus 6M," InterCAT Technical Workgroup Meeting, APS, IL, July 18, 2013.

    Kanagalaghatta Rajashankar, "Behind the beamstop crystallography," APS Life Science Council R&D Workshop. Argonne Guest House, Argonne, IL, May 2, 2012.

    Jonathan Schuermann, “APS Status Report,” Kappa Meeting, 2013, Lund, Sweden, April 16 and 17, 2013.

    Jonathan Schuermann, “RAPD,” Kappa Meeting, 2013, Lund, Sweden, April 16 and 17, 2013.

    Kanagalaghatta Rajashankar, “Better Detector; Better Data,” Annual Meeting of the P41 Principal Investigator Meeting, “Tackling More Challenging Biomedical Research Needs With New Consortia of P41 Centers,” Rockville, MD, March 18 and 19, 2013.

    Poster

    Kay Perry, Malcolm Capel, Surajit Banerjee, Igor Kourinov, Ed Lynch, Frank Murphy, David Neau, Kanagalaghatta Rajashankar, Cynthia Salbego, Jonathan Schuermann, Narayanasami Sukumar, James Withrow & Steven E. Ealick, “NorthEastern Collaborative Access Team (NE-CAT) Beam Lines at the Advanced Photon Source” 2013 Annual Meeting of the American Crystallographic Association, Honolulu, Hawaii, July  20 – July 24, 2013.

    2012 Staff Activities

    Publications

    Xu, K., Chan, Y. P., Rajashankar, K. R., Khetawat, D., Yan, L., Kolev, M. V., Broder, C. C., and Nikolov, D. B. (2012) New insights into the hendra virus attachment and entry process from structures of the virus g glycoprotein and its complex with ephrin-β2, PLoS One 7, e48742. PubMed

    Kaiser, S. E., Mao, K., Taherbhoy, A. M., Yu, S., Olszewski, J. L., Duda, D. M., Kurinov, I., Deng, A., Fenn, T. D., Klionsky, D. J., and Schulman, B. A. (2012) Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7-Atg10 structures, Nat. Struct. Mol. Biol. 19, 1242-1249. PubMed

    Ketkar, A., Zafar, M. K., Marquez, V. E., Banerjee, S., Egli, M., and Eoff, R. L. (2012) Differential furanose selection in the active sites of archaeal DNA polymerases probed by fixed-conformation nucleotide analogues, Biochemistry 51, 9234−9244. PubMed

    Cavalier, M. C., Yim, Y. S., Asamizu, S., Neau, D., Almabruk, K. H., Mahmud, T., and Lee, Y. H. (2012) Mechanistic Insights into Validoxylamine A 7'-Phosphate Synthesis by VldE Using the Structure of the Entire Product Complex, PLoS One 7, e44934. PubMed

    Martin, R., Gupta, K., Ninan, N. S., Perry, K., and Van Duyne, G. D. (2012) The Survival Motor Neuron Protein Forms Soluble Glycine Zipper Oligomers, Structure. 20, 1929–1939. PubMed

    Windsor, M. A., Hermanson, D. J., Kingsley, P. J., Xu, S., Crews, B. C., Ho, W., Keenan, C. M., Banerjee, S., Sharkey, K. A., and Marnett, L. J. (2012) Substrate-Selective Inhibition of Cyclooxygenase-2: Development and Evaluation of Achiral Profen Probes, ACS Med. Chem. Lett. 3, 759–763. PubMed

    Logsdon, N. J., Deshpande, A., Harris, B. D., Rajashankar, K. R., and Walter, M. R. (2012) Structural basis for receptor sharing and activation by interleukin-20 receptor-2 (IL-20R2) binding cytokines, Proc. Natl. Acad. Sci. U. S. A. 109, 12704-12709. PubMed

    Bolla, J. R., Do, S. V., Long, F., Dai, L., Su, C. C., Lei, H. T., Chen, X., Gerkey, J. E., Murphy, D. C., Rajashankar, K. R., Zhang, Q., and Yu, E. W. (2012) Structural and functional analysis of the transcriptional regulator Rv3066 of Mycobacterium tuberculosis, Nucleic Acids Res. 40, 9340-9355. PubMed

    Su, C. C., Long, F., Lei, H. T., Bolla, J. R., Do, S. V., Rajashankar, K. R., and Yu, E. W. (2012) Charged Amino Acids (R83, E567, D617, E625, R669, and K678) of CusA Are Required for Metal Ion Transport in the Cus Efflux System, J. Mol. Biol. 422, 429–441. PubMed

    Banerjee, S., Christov, P. P., Kozekova, A., Rizzo, C. J., Egli, M., and Stone, M. P. (2012) Replication bypass of the trans-4-Hydroxynonenal-derived (6S,8R,11S)-1,N2-deoxyguanosine DNA adduct by the Sulfolobus solfataricus DNA polymerase IV, Chem. Res. Toxicol. 25, 422-435. PubMed (Data not taken at NE-CAT)

    Ketkar, A., Zafar, M. K., Banerjee, S., Marquez, V. E., Egli, M., and Eoff, R. L. (2012) A nucleotide analogue induced gain of function corrects the error-prone nature of human DNA polymerase iota, J. Am. Chem. Soc. 134, 10698–10705. PubMed

    Ren, A., Rajashankar, K. R., and Patel, D. J. (2012) Fluoride ion encapsulation by Mg2+ ions and phosphates in a fluoride riboswitch, Nature 486, 85-89. PubMed

    Gilbert, N. C., Rui, Z., Neau, D. B., Waight, M. T., Bartlett, S. G., Boeglin, W. E., Brash, A. R., and Newcomer, M. E. (2012) Conversion of human 5-lipoxygenase to a 15-lipoxygenase by a point mutation to mimic phosphorylation at Serine-663, FASEB J. 26,3222-3229. PubMed

    Lai, R. Y., Huang, S., Fenwick, M. K., Hazra, A. B., Zhang, Y., Rajashankar, K. R., Philmus, B., Kinsland, C., Sanders, J. M., Ealick, S. E., and Begley, T. P. (2012) Thiamin pyrimidine biosynthesis in Candida albicans: a remarkable reaction between histidine and pyridoxal phosphate, J. Am. Chem. Soc. 134, 9157−9159. PubMed

    Chan, R. T., Robart, A. R., Rajashankar, K. R., Pyle, A. M., and Toor, N. (2012) Crystal structure of a group II intron in the pre-catalytic state, Nat. Struct. Mol. Biol. 19, 555-557. PubMed

    Stokes-Rees, I., Levesque, I., Murphy, F. V. t., Yang, W., Deacon, A., and Sliz, P. (2012) Adapting federated cyberinfrastructure for shared data collection facilities in structural biology, J. Synchrotron Radiat. 19, 462-467. PubMed

    Zhao, B., Lei, L., Kagawa, N., Sundaramoorthy, M., Banerjee, S., Nagy, L. D., Guengerich, F. P., and Waterman, M. R. (2012) A Three-dimensional Structure of Steroid 21-Hydroxylase (Cytochrome P450 21A2) with Two Substrates Reveals Locations of Disease-associated Variants. J. Biol. Chem. 287, 10613-10622. PubMed (data not taken at the Resource)

    Ekworomadu, M. T., Poor, C. B., Owens, C. P., Balderas, M. A., Fabian, M., Olson, J. S., Murphy, F., Balkabasi, E., Honsa, E. S., He, C., Goulding, C. W., and Maresso, A. W. (2012) Differential Function of Lip Residues in the Mechanism and Biology of an Anthrax Hemophore. PLoS Pathog. 8, e1002559. PubMed

    Cavalier, M. C., Kim, S. G., Neau, D., and Lee, Y. H. (2012) Molecular basis of the fructose-2,6-bisphosphatase reaction of PFKFB3: Transition state and the C-terminal function, Proteins 80, 1143-1153. PubMed

    Gu, S., Rumpel, S., Zhou, J., Strotmeier, J., Bigalke, H., Perry, K., Shoemaker, C. B., Rummel, A., and Jin, R. (2012) Botulinum Neurotoxin Is Shielded by NTNHA in an Interlocked Complex, Science 335, 977-981. PubMed

    Zong, Y., Zhang, B., Gu, S., Lee, K., Zhou, J., Yao, G., Figueiredo, D., Perry, K., Mei, L., and Jin, R. (2012) Structural basis of agrin-LRP4-MuSK signaling, Genes Dev. 26, 247-258. PubMed

    Vendeix, F. A., Murphy, F. V. t., Cantara, W. A., Leszczynska, G., Gustilo, E. M., Sproat, B., Malkiewicz, A., and Agris, P. F. (2012) Human tRNA(Lys3)(UUU) Is Pre-Structured by Natural Modifications for Cognate and Wobble Codon Binding through Keto-Enol Tautomerism, J. Mol. Biol. 416, 467-485 PubMed

    Logsdon, N. J., Allen, C. E., Rajashankar, K. R., and Walter, M. R. (2012) Purification, crystallization and preliminary X-ray diffraction analysis of the IL-20-IL-20R1-IL-20R2 complex, Acta Crystallogr. F 68, 89-92. PubMed

    Li, J., Malakhova, M., Mottamal, M., Reddy, K., Kurinov, I., Carper, A., Langfald, A., Oi, N., Kim, M. O., Zhu, F., Sosa, C. P., Zhou, K., Bode, A. M., and Dong, Z. (2012) Norathyriol suppresses skin cancers induced by solar ultraviolet radiation by targeting ERK kinases, Cancer Res.72, 260-270. PubMed

    Presentations

    Narayanasami Sukumar. Plenary lecture "Crystallographic studies on cobalamin binding proteins in eukaryotes and prokaryotes" at the Vitamin B12 Symposium, Faculte de Medecine, University of Lorraine, Nancy, France, September 20-22, 2012.

    Narayanasami Sukumar. Co-Chair of section on "Structure-based molecular characteristics and biochemistry of vitamin B12 enzymes"at the Vitamin B12 Symposium, Faculte de Medecine, University of Lorraine, Nancy, France, September 20-22, 2012.

    Malcolm Capel, Kanagalaghatta, Rajashankar, Frank Murphy, Steve Ealick. "Integration of the Dectris Pilatus 6MF PAD detector at NE-CAT". Annual Meeting of the American Crystallographic Association, Boston, MA, July 28 - August 1, 2012.

    Raj Rajashankar, Igor Kourinov, Malcolm Capel, Steve Ealick. "Data Collection with 6 Million Detectors. " Annual Meeting of the American Crystallographic Association, Boston, MA, July 28 - August 1, 2012.

    Surajit Banerjee, Kanagalaghatta Rajashankar. "When to do a MAD Experiment? " Annual Meeting of the American Crystallographic Association, Boston, MA, July 28 - August 1, 2012.

    Jonathan P. Schuermann, Frank V. Murphy, "NE-CAT Remote Access", 18th Annual Harvard Structural Biology Retreat, American Academy of Arts and Sciences, Cambridge, MA. July 26, 2012.

    Jonathan P. Schuermann, "RAPD - Rapid Automated Processing of Data", DIALS - Partnering Data Collection and Reduction in the Beamline Environment. Harvard Medical School, Boston, MA. July 27, 2012.

    Poster Sessions

    Surajit Banerjee, Amit Ketkar, Maroof Zafar, Victor Marquez, Martin Egli, Robert Eoff. "Conformationally Restrained North-methanocarba- 2'-deoxyadenosine Corrects the Error- Prone Nature of Human DNA Polymerase Iota." Annual Meeting of the American Crystallographic Association, Boston, MA, July 28 - August 1, 2012.

    Igor Kourinov, Malcolm Capel, Surajit Banerjee, Frank Murphy, David Neau, Kay Perry, Kanagalaghatta Rajashankar, Jonathan Schuermann, Narayanasami Sukumar, Steven E. Ealick. "NE-CAT Crystallography Beamlines for Challenging Structural Biology Research. " Annual Meeting of the American Crystallographic Association, Boston, MA, July 28 - August 1, 2012.

    Kay Perry, Tadhg Begley, Steven Ealick. "Structural Study of Isonitrile Synthase." Annual Meeting of the American Crystallographic Association, Boston, MA, July 28 - August 1, 2012.

    Narayanasami Sukumar. "A comprehensive review on B12 binding proteins." Gordon Research Conference, Diffraction Methods in Structural Biology, Lewiston, ME, July 15 - July 20, 2012.

    2011 Staff Activities

    Publications

    Taherbhoy, A. M., Tait, S. W., Kaiser, S. E., Williams, A. H., Deng, A., Nourse, A., Hammel, M., Kurinov, I., Rock, C. O., Green, D. R., and Schulman, B. A. (2011) Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway, Mol. Cell 44, 451-461. PubMed

    Duggan, K. C., Hermanson, D. J., Musee, J., Prusakiewicz, J. J., Scheib, J. L., Carter, B. D., Banerjee, S., Oates, J. A., and Marnett, L. J. (2011) (R)-Profens are substrate-selective inhibitors of endocannabinoid oxygenation by COX-2, Nat. Chem. Biol. 7, 803-809. PubMed

    Seo, M., Kim, J. D., Neau, D., Sehgal, I., and Lee, Y. H. (2011) Structure-Based Development of Small Molecule PFKFB3 Inhibitors: A Framework for Potential Cancer Therapeutic Agents Targeting the Warburg Effect, PLoS One 6, e24179. PubMed

    Khare, B., Krishnan, V., Rajashankar, K. R., I-Hsiu, H., Xin, M., Ton-That, H., and Narayana, S. V. (2011) Structural Differences between the Streptococcus agalactiae Housekeeping and Pilus-Specific Sortases: SrtA and SrtC1, PLoS One 6, e22995. PubMed

    Sukumar, N., Choi, M., and Davidson, V. L. Replacement of the axial copper ligand methionine with lysine in amicyanin converts it to a zinc-binding protein that no longer binds copper, J. Inorg. Biochem. 105, 1638-1644. PubMed

    Calabrese, M. F., Scott, D. C., Duda, D. M., Grace, C. R., Kurinov, I., Kriwacki, R. W., and Schulman, B. A. (2011) A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases, Nat. Struct. Mol. Biol. 14, 947-949. PubMed

    Nam, K. H., Kurinov, I., and Ke, A. (2011) Crystal Structure of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated Csn2 Protein Revealed Ca2+-dependent Double-stranded DNA-binding Activity, J. Biol. Chem. 286, 30759-30768. PubMed

    Peng, G., Sun, D., Rajashankar, K. R., Qian, Z., Holmes, K. V., and Li, F. (2011) Crystal structure of mouse coronavirus receptor-binding domain complexed with its murine receptor, Proc. Natl. Acad. Sci. U. S. A. 108, 10696-10701. PubMed

    French, J. B., Neau, D. B., and Ealick, S. E. (2011) Characterization of the Structure and Function of Klebsiella pneumoniae Allantoin Racemase, J. Mol. Biol. 410, 447-460. PubMed

    Larson, M. R., Rajashankar, K. R., Crowley, P. J., Kelly, C., Mitchell, T. J., Brady, L. J., and Deivanayagam, C. (2011) Crystal structure of the C-terminus of Streptococcus mutans antigen I/II and characterization of salivary agglutinin adherence domains, J. Biol. Chem. 286, 21657-21666. PubMed

    Ding, F., Lu, C., Zhao, W., Rajashankar, K. R., Anderson, D. L., Jardine, P. J., Grimes, S., and Ke, A. (2011) Structure and assembly of the essential RNA ring component of a viral DNA packaging motor, Proc. Natl. Acad. Sci. U. S. A. 108, 7357-7362. PubMed

    Cao, Y., Jin, X., Levin, E. J., Huang, H., Zong, Y., Quick, M., Weng, J., Pan, Y., Love, J., Punta, M., Rost, B., Hendrickson, W. A., Javitch, J. A., Rajashankar, K. R., and Zhou, M. (2011) Crystal structure of a phosphorylation-coupled saccharide transporter, Nature 473, 50-54. PubMed

    Cao, Y., Jin, X., Huang, H., Derebe, M. G., Levin, E. J., Kabaleeswaran, V., Pan, Y., Punta, M., Love, J., Weng, J., Quick, M., Ye, S., Kloss, B., Bruni, R., Martinez-Hackert, E., Hendrickson, W. A., Rost, B., Javitch, J. A., Rajashankar, K. R., Jiang, Y., and Zhou, M. (2011) Crystal structure of a potassium ion transporter, TrkH, Nature 471, 336-340. PubMed

    Su, C.-C., Long, F., Zimmermann, M. T., Rajashankar, K. R., Jernigan, R. L., and Yu, E. W. (2011) Crystal structure of the CusBA heavy-metal efflux complex of Escherichia coli, Nature 470, 558-562. PubMed

    Lombardi, P. M., Angell, H. D., Whittington, D. A., Flynn, E. F., Rajashankar, K. R., and Christianson, D. W. (2011) Structure of Prokaryotic Polyamine Deacetylase Reveals Evolutionary Functional Relationships with Eukaryotic Histone Deacetylases, Biochemistry 50, 1808-1817. PubMed

    Gilbert, N. C., Bartlett, S. G., Waight, M. T., Neau, D. B., Boeglin, W. E., Brash, A. R., and Newcomer, M. E. (2011) The structure of human 5-lipoxygenase, Science 331, 217-219. PubMed

    Choi, M., Sukumar, N., Mathews, F. S., Liu, A., and Davidson, V. L. (2011) Proline 96 of the Copper Ligand Loop of Amicyanin Regulates Electron Transfer from Methylamine Dehydrogenase by Positioning Other Residues at the Protein-Protein Interface, Biochemistry 50, 1265-1273. PubMed

    Presentations

    Narayanasami Sukumar. Plenary lecture "Crystallographic studies on cobalamin binding proteins in eukaryotes and prokaryotes" at the Vitamin B12 Symposium, Faculte de Medecine, University of Lorraine, Nancy, France, September 20-22, 2012.

    Narayanasami Sukumar. Co-Chair of section on "Structure-based molecular characteristics and biochemistry of vitamin B12 enzymes"at the Vitamin B12 Symposium, Faculte de Medecine, University of Lorraine, Nancy, France, September 20-22, 2012.

    Narayansami Sukumar, "Analysis of Electron Transfer Reaction in Type I Copper Protein", invited lecture at the Center for Advance Studies in Crystallography and Biophysics, University of Madras, Chennai, India, August 19, 2011.

    Kanagalaghatta Rajashankar, “Microdiffraction: What can be done?” INDO-US Workshop/Symposium on Modern Trends in Macromolecular Structures, Indian Institute of Technology Bombai, Mumbai-400076, India, February 21-24, 2011.

    Kanagalaghatta Rajashankar, “Workshop on Macromolecular Structure Determination (Advanced Level)” INDO-US Workshop/Symposium on Modern Trends in Macromolecular Structures, Indian Institute of Technology Bombai, Mumbai-400076, India, February 21-24, 2011.

    Malcolm Capel and Frank Murphy, “Vector Scanned Microcrystallographic Data Collection Techniques,” 2011 Annual Meeting of the American Crystallographic Association, New Orleans, Louisiana, May 28 – June 2, 2011.

    Narayanasami Sukumar, “Defining the role of the axial ligand of the type 1 copper site in amicyanin, an electron transfer protein from Paracoccus denitrificans,” Annual Meeting of the American Crystallographic Association, New Orleans, Louisiana, May 28 – June 2, 2011

    Frank Murphy, What's New at NE-CAT? Harvard University, June 22, 2011

    Poster Sessions

    Surajit Banerjee, Roberto Vanacore, M. Sundaramoorthy, Ariel Boutaud, Billy G. Hudson. "Crystal Structure of Type IV Collagen α-2 NC1 Domain Monomer", Collagen Gordon Research Conference, Colby-Sawyer College, New London, NH, July 17-22, 2011

    Surajit Banerjee, Kelsey C. Duggan, Daniel J. Hermanson, Joel Muse, Jeffery J. Prusakiewicz, Jami L. Scheib, Bruce D. Carter, John A. Oates, Lawrence J. Marnett, "Structural Insights into the Binding Mechanism of (R)- and (S)-Naproxen with Cyclooxygenase-2," Annual Meeting of the American Crystallographic Association, New Orleans, Louisiana, May 28 – June 2, 2011.

    Kanagalaghatta Rajashankar , Steven Ealick, Malcolm Capel, Surajit Banerjee, Amit Belani, Anthony Lynch, Frank Murphy, Igor Kourinov, David Neau, Kay Perry, Cynthia Salbego, Jonathan Schuermann, Narayanasami Sukumar, James Withrow, "Northeastern CAT Crystallography Beam Lines for Challenging Structural Biology Research," Annual Meeting of the American Crystallographic Association, New Orleans, Louisiana, May 28 – June 2, 2011.

    2010 Staff Activities

    Demirci, H., Murphy, F. V. IV, Belardinelli, R., Kelley, A. C., Ramakrishnan, V., Gregory, S. T., Dahlberg, A. E., and Jogl, G. (2010) Modification of 16S ribosomal RNA by the KsgA methyltransferase restructures the 30S subunit to optimize ribosome function, RNA 16, 2319-2324. PubMed

    Singh, H., Schuermann, J. P., Reilly, T. J., Calcutt, M. J., and Tanner, J. J. (2010) Recognition of Nucleoside Monophosphate Substrates by Haemophilus influenzae Class C Acid Phosphatase, J. Mol. Biol. 404, 639-649. PubMed

    Bale, S., Rajashankar, K. R., Perry, K., Begley, T. P., and Ealick, S. E. (2010) HMP Binding Protein ThiY and HMP-P Synthase THI5 are Structural Homologues, Biochemistry 49, 8929-8936. PubMed

    Long, F., Su, C.-C., Zimmermann, M. T., Boyken, S. E., Rajashankar, K. R., Jernigan, R. L., and Yu, E. W. (2010) Crystal structures of the CusA efflux pump suggest methionine-mediated metal transport, Nature 467, 484-488. PubMed

    Khare, B., Samal, A., Vengadesan, K., Rajashankar, K. R., Ma, X., Huang, I. H., Ton-That, H., and Narayana, S. V. (2010) Preliminary crystallographic study of the Streptococcus agalactiae sortases, sortase A and sortase C1, Acta Crystallogr. F 66, 1096-1100. PubMed

    Himanen, J. P., Yermekbayeva, L., Janes, P. W., Walker, J. R., Xu, K., Atapattu, L., Rajashankar, K. R., Mensinga, A., Lackmann, M., Nikolov, D. B., and Dhe-Paganon, S. (2010) Architecture of Eph receptor clusters, Proc. Natl. Acad. Sci. U. S. A. 107, 10860-10865. PubMed

    Kovalevsky, A. Y., Fisher, S. Z., Seaver, S., Mustyakimov, M., Sukumar, N., Langan, P., Mueser, T. C., and Hanson, B. L. (2010) Preliminary neutron and X-ray crystallographic studies of equine cyanomethemoglobin, Acta Crystallogr. F 66, 474-477. PubMed

    Sukumar, N., Mathews, F. S., Langan, P., and Davidson, V. L. (2010) A joint x-ray and neutron study on amicyanin reveals the role of protein dynamics in electron transfer, Proc. Natl. Acad. Sci. U. S. A. 107, 6817-6822. PubMed

    Malakhova, M., D'Angelo, I., Kim, H. G., Kurinov, I., Bode, A. M., and Dong, Z. (2010) The crystal structure of the active form of the C-terminal kinase domain of mitogen-and stress-activated protein kinase 1. J. Mol. Biol. 399, 41-52. PubMed

    Paul, D., O'Leary, S. E., Rajashankar, K., Bu, W., Toms, A., Settembre, E. C., Sanders, J. M., Begley, T. P., and Ealick, S. E. (2010) Glycal Formation in Crystals of Uridine Phosphorylase. Biochemistry 49, 3499-3509. PubMed

    Larson, M. R., Rajashankar, K. R., Patel, M. H., Robinette, R. A., Crowley, P. J., Michalek, S., Brady, L. J., and Deivanayagam, C. (2010) Elongated fibrillar structure of a streptococcal adhesin assembled by the high-affinity association of α- and PPII-helices, Proc. Natl. Acad. Sci. U. S. A. 107, 5983-5988. PubMed

    Gu, M., Rajashankar, K. R., and Lima, C. D. (2010) Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA Capping Apparatus, Structure 18, 216-227. PubMed

    Schuermann, J. P., Tan, A., Tanner, J. J., and Henzl, M. T. (2010) Structure of Avian Thymic Hormone, a High-affinity Avian β-parvalbumin, in the Ca2+-free and Ca2+-bound States, J. Mol. Biol. 397, 991-1002. PubMed

    Srivastava, D., Schuermann, J. P., White, T. A., Krishnan, N., Sanyal, N., Hura, G. L., Tan, A., Henzl, M. T., Becker, D. F., and Tanner, J. J. (2010) Crystal structure of the bifunctional proline utilization A flavoenzyme from Bradyrhizobium japonicum, Proc. Natl. Acad. Sci. U. S. A. 107, 2878-2883. PubMed

    Perry, K., Hwang, Y., Bushman, F. D., and Van Duyne, G. D. (2010) Insights from the Structure of a Smallpox Virus Topoisomerase-DNA Transition State Mimic, Structure 18, 127-137. PubMed

    Toor, N., Keating, K. S., Fedorova, O., Rajashankar, K., Wang, J., and Pyle, A. M. (2010) Tertiary architecture of the Oceanobacillus iheyensis group II intron, RNA16, 57-69. PubMed

    2009 Staff Activities

    Publications

    Malakhova, M., Kurinov, I., Liu, K., Zheng, D., D'Angelo, I., Shim, J. H., Steinman, V., Bode, A. M., and Dong, Z. (2009) Structural diversity of the active N-terminal kinase domain of p90 ribosomal S6 kinase 2, PLoS One 4, e8044. PubMed

    Singh, H., Felts, R. L., Schuermann, J. P., Reilly, T. J., and Tanner, J. J. (2009) Crystal Structures of the Histidine Acid Phosphatase from Francisella tularensis Provide Insight into Substrate Recognition, J. Mol. Biol. 394, 893-904. PubMed

    Schmeing, T. M., Voorhees, R. M., Kelley, A. C., Gao, Y. G., Murphy, F. V. t., Weir, J. R., and Ramakrishnan, V. (2009) The Crystal Structure of the Ribosome Bound to EF-Tu and Aminoacyl-tRNA, Science 326, 688-694. PubMed

    Galaleldeen, A., Strange, R., Whitson, L. J., Antonyuk, S., Narayana, N., Taylor, A. B., Schuermann, J. P., Holloway, S. P., Hasnain, S. S., and Hart, P. J. (2009) Structural and Biophysical Properties of Metal-Free Pathogenic SOD1 Mutants A4V and G93A, Arch Biochem. Biophys. 492, 40-47. PubMed

    Wiltzius, J. J., Landau, M., Nelson, R., Sawaya, M. R., Apostol, M. I., Goldschmidt, L., Soriaga, A. B., Cascio, D., Rajashankar, K., and Eisenberg, D. (2009) Molecular mechanisms for protein-encoded inheritance, Nat. Struct. Mol. Biol. 16, 973-978. PubMed

    Wang, J., Dye, B. T., Rajashankar, K. R., Kurinov, I., and Schulman, B. A. (2009) Insights into anaphase promoting complex TPR subdomain assembly from a CDC26-APC6 structure, Nat. Struct. Mol. Biol. 16, 987-989. PubMed

    Choi, M., Sukumar, N., Liu, A., and Davidson, V. L. (2009) Defining the Role of the Axial Ligand of the Type 1 Copper Site in Amicyanin by Replacement of Methionine with Leucine, Biochemistry 48, 9174-9184.PubMed

    Neau, D. B., Gilbert, N. C., Bartlett, S. G., Boeglin, W., Brash, A. R., and Newcomer, M. E. (2009) The 1.85 Å Structure of an 8R-Lipoxygenase Suggests a General Model for Lipoxygenase Product Specificity, Biochemistry 48, 7906-7915. PubMed

    Su, C.-C., Yang, F., Long, F., Reyon, D., Routh, M. D., Kuo, D. W., Mokhtari, A. K., Van Ornam, J. D., Rabe, K. L., Hoy, J. A., Lee, Y. J., Rajashankar, K. R., and Yu, E. W. Crystal structure of the membrane fusion protein CusB from Escherichia coli, J. Mol. Biol. 393, 342-355. PubMed

    Schuette, J. C., Murphy, F. V. IV, Kelley, A. C., Weir, J. R., Giesebrecht, J., Connell, S. R., Loerke, J., Mielke, T., Zhang, W., Penczek, P. A., Ramakrishnan, V., and Spahn, C. M. (2009) GTPase activation of elongation factor EF-Tu by the ribosome during decoding, EMBO J. 28, 755-765. PubMed

    Wang, Q., Navarro, M. V., Peng, G., Molinelli, E., Shih, L. G., Judson, B. L., Rajashankar, K. R., and Sondermann, H. (2009) Molecular mechanism of membrane constriction and tubulation mediated by the F-BAR protein Pacsin/Syndapin, Proc. Natl. Acad. Sci. U. S. A. 106, 12700-12705. PubMed

    Sukumar, N., Mathews, F. S., Gordon, M. M., Ealick, S. E., and Alpers, D. H. (2009) Postcrystallization Analysis of the Irreproducibility of the Human Intrinsic Factor-Cobalamin Complex Crystals, Cryst. Growth Des. 9, 348-351. PubMed

    Himanen, J. P., Goldgur, Y., Miao, H., Myshkin, E., Guo, H., Buck, M., Nguyen, M., Rajashankar, K. R., Wang, B., and Nikolov, D. B. (2009) Ligand recognition by A-class Eph receptors: crystal structures of the EphA2 ligand-binding domain and the EphA2/ephrin-A1 complex, EMBO Rep. 10, 722-728. PubMed

    Sukumar, N., Dewanti, A., Merli, A., Rossi, G. L., Mitra, B., and Mathews, F. S. (2009) Structures of the G81A mutant form of the active chimera of (S)-mandelate dehydrogenase and its complex with two of its substrates, Acta Crystallogr. D 65, 543-552. PubMed

    Bick, M. J., Lamour, V., Rajashankar, K. R., Gordiyenko, Y., Robinson, C. V., and Darst, S. A. (2009) How to Switch Off a Histidine Kinase: Crystal Structure of Geobacillus stearothermophilus KinB with the inhibitor Sda, J. Mol. Biol. 386, 163-177. PubMed

    Ostrander, E. L., Larson, J. D., Schuermann, J. P., and Tanner, J. J. (2009) A Conserved Active Site Tyrosine Residue of Proline Dehydrogenase Helps Enforce the Preference for Proline over Hydroxyproline as the Substrate Biochemistry 48, 951-959. PubMed

    Presentations

    Use of Micro-focused Beamline in Solving the Crystal Structure of the Avian Thymic Hormone (ATH) in Calcium Bound Form, Jon Schuermann, 2009 ACA Meeting, Toronto CA, July 25-30, 2009.

    Malcolm Capel, March 28 2009 EMBL-ESRF: Development of the MD2 microdiffractometer at the APS-NECAT beamlines.

    Malcolm Capel, "Recent Operating and Development Experiences with the NE-CAT ACCEL/Maatel MD2 Microdiffractometer", APS Technical Working Group (TWG) Meeting, April 16, 2009.

    Poster Sessions

    Microdiffraction-What Can be Done?, K. Rajashankar, M. Capel, S. Harrison, and S. Ealick, 2009 ACA Meeting, Toronto CA, July 25-30, 2009

    Northeastern Collaborative Access Team ( NE- CAT) Beamlines at the Advanced Photon Source, I. Kourinov, S. Ealick, M. Capel, F. Murphy,   D. Neau, K. Perry, K. Rajashankar, J. Schuermann, and N. Sukumar, 2009 ACA Meeting, Toronto CA, July 25-30, 2009

    Committee Memberships

    K. Rajashankar, APS Beamtime Allocation Committee

    M. Capel, BioCAT Resource Advisory Committee

    M. Capel, Partner User Council (PUG)

    Malcolm Capel, Design Committee of the Proteins to Organisms Imaging Beamline, Renewal of the Advanced Photon Source Project

    2008 Staff Activities

    Publications

    Ostrander, E. L., Larson, J. D., Schuermann, J. P., and Tanner, J. J. (2009) A Conserved Active Site Tyrosine Residue of Proline Dehydrogenase Helps Enforce the Preference for Proline over Hydroxyproline as the Substrate Biochemistry 48, 951-959. PubMed

    Brohawn, S. G., Leksa, N. C., Spear, E. D., Rajashankar, K. R., and Schwartz, T. U. (2008) Structural Evidence for Common Ancestry of the Nuclear Pore Complex and Vesicle Coats, Science 322, 1369-1373 PubMed

    Toor, N., Rajashankar, K., Keating, K. S., and Pyle, A. M. (2008) Structural basis for exon recognition by a group II intron, Nat. Struct. Mol. Biol. 15, 1221-1222. PubMed

    Mao, D. Y., Neculai, D., Downey, M., Orlicky, S., Haffani, Y. Z., Ceccarelli, D. F., Ho, J. S., Szilard, R. K., Zhang, W., Ho, C. S., Wan, L., Fares, C., Rumpel, S., Kurinov, I., Arrowsmith, C. H., Durocher, D., and Sicheri, F. (2008) Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine, Mol. Cell 32, 259-275. PubMed

    Xu, K., Rajashankar, K. R., Chan, Y. P., Himanen, J. P., Broder, C. C., and Nikolov, D. B. (2008) Host cell recognition by the henipaviruses: crystal structures of the Nipah G attachment glycoprotein and its complex with ephrin-B3, Proc. Natl. Acad. Sci. U. S. A. 105, 9953-9958. PubMed

    Rajakulendran, T., Sahmi, M., Kourinov, I., Tyers, M., Therrien, M., and Sicheri, F. (2008) CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling, Proc. Natl. Acad. Sci. U. S. A. 105, 2836-2841. PubMed

    Soriano, E. V., Rajashankar, K. R., Hanes, J. W., Bale, S., Begley, T. P., and Ealick, S. E. (2008) Structural Similarities between Thiamin-Binding Protein and Thiaminase-I Suggest a Common Ancestor, Biochemistry 47, 1346-1357. PubMed

    Lin, S. C., Chung, J. Y., Lamothe, B., Rajashankar, K., Lu, M., Lo, Y. C., Lam, A. Y., Darnay, B. G., and Wu, H. (2008) Molecular Basis for the Unique Deubiquitinating Activity of the NF- kB Inhibitor A20, J. Mol. Biol. 376, 526-540. PubMed

    Hlinkova, V., Xing, G., Bauer, J., Shin, Y. J., Dionne, I., Rajashankar, K. R., Bell, S. D., and Ling, H. (2008) Structures of monomeric, dimeric and trimeric PCNA: PCNA-ring assembly and opening, Acta Crystallogr. D 64, 941-949. PubMed

    Weixlbaumer and F. Murphy”Crystallographic Studies of Decoding by Modified Bases: Correlation of Structure and Function”, in “ DNA and RNA Modification Enzmes: Comparative Structure, Mechanism, Functions, Cellular Interactions and Evolution:. Editor Henri Grosjean, To be published Landes Bioscience, 2009.

    Presentations

    Dealing With Non-Merohedral Twinning, K, Rajashanker, ACA 2008, Knoxville, TN, May 31-June 5

    SAD Data Collection and Experimental Setup, K. Rajashankar, 66 th Annual Pittsburg Diffraction Conference, October 30, 2008

    Crystallographic Studies on a Vitamin B12 Transport Protein and an Electronic Transfer Complex, N. Sukumar ANL Biosciences Division Seminar, September 25, 2008

    Poster Presentations

    K. Rajashankar, Microdiffraction: What Can Be Done? APS User Week 2007

    K. Rajashankar, Microdiffraction: What Can Be Done? NCRR Biomedical Technology Centers Principal Investigator Meeting, , 2008

    I. Kourinov et al, Implementation of Microdiffraction Techniques at Northeastern CAT Beamlines, International Union of Crystallography, Osaka, Japan 2008

    F. Murphy, “Alloy- Data Processing for Multi-Crystal Macromolecular Crystallography, APS User Week 2008

    Workshops Organized

    Engage Your Brain, K. Rajashankar and Z. Dauter, ACA 2008, Knoxville, TN May 31-June 5

    Software for Challenging Cases in Macromolecular Crystallography, I. Kourinov and F. Murphy, APS User Week May 2008

    Memberships and Awards

    K. Rajashankar, APS Beamtime Allocation Committee

    J. Unik, Outstanding Alumnus 2008, Biological, Chemical, and Physics Sciences Department, Illinois Institute of Technology