Primary funding for this project comes from the National Institute of General Medical Sciences(NIGMS) , a division of the National Institutes of Health (NIH). Additional financial support for NE-CAT comes from the member institutions.
National Institutes of Health
Status of NE-CAT Sector 24 Activities
The accelerator was shut down for scheduled preventative maintenance for nearly the entire month of May. These long accelerator shutdown periods, which occur three times a year, are used by the NE- CAT staff to perform their own preventative maintenance on the beamlines and to install and test new capabilities for its users.
The major emphasis during this shutdown period was to develop a new software interface between the Console beamline control system and the MD2 microdiffractometer control system. When delivered, the MD2 was supplied with its own control system and Maatel had also provided an interface to enable the MD2 to communicate with an external control system. However in usage, our users would periodically experience a hang up requiring rebooting of the system due to problems with the supplied interface. Also the communications between the Console control system and the MD2 software through the supplied interface were very slow, appreciably slowing down the automated sample placement robot operation as well as the rate at which data frames could be taken. To alleviate this problem, a new interface software system was developed and installed during the shutdown period. Following resumption of APS accelerator operations on May 27, our staff thoroughly tested the new interface before arrival of the first users. The new interface was found to be highly reliable; totally eliminating the hang up problems previously encountered and permitted must faster operations. The complete cycle of pin dismount and new pin remount on the goniometer using the sample placement robot was decreased from 2-1/2 minutes to 1-1/4 minutes. Also, the rate at which data frames could be taken was increased by a factor of two. Based upon the successful testing of the new interface, this new control interface has now been installed on both beamlines for routine operations.
Several other new features have been added. A triple aperture assembly has been purchased from ACCEL/Maatel. This assembly contains three different sized apertures. From the control area users can now automatically select which of the three aperture sizes they wish to use- avoiding the need to go in and out of the experimental hutch to manually change apertures. A MD2 Kappa attachment for the MD2 is now available for routine use by our users on the 24-ID-C beamline. To facilitate use of the Kappa, we have made available to the users the STAC (Strategy for Aligned Crystals) software. STAC allows the user to automatically determine the correct orientation of the crystal and provides an optimum data acquisition strategy to be used.
January 15, 2015
Lin, J., Gagnon, M. G., Bulkley, D., and Steitz, T. A. (2015) Conformational Changes of Elongation Factor G on the Ribosome during tRNA Translocation, Cell 160, 219-227.
January 9, 2015
Leung, J. H., Schurig-Briccio, L. A., Yamaguchi, M., Moeller, A., Speir, J. A., Gennis, R. B., and Stout, C. D. (2015) Structural biology. Division of labor in transhydrogenase by alternating proton translocation and hydride transfer, Science 347, 178-181.
January 1, 2015
Li, X., Roberti, R., and Blobel, G. (2015) Structure of an integral membrane sterol reductase from Methylomicrobium alcaliphilum, Nature 517, 104-107.
December 19, 2014
Joh, N. H., Wang, T., Bhate, M. P., Acharya, R., Wu, Y., Grabe, M., Hong, M., Grigoryan, G., and DeGrado, W. F. (2014) De novo design of a transmembrane Zn2+-transporting four-helix bundle, Science 346, 1520-1524.
Highlighted in Lupas, A. N. (2014) Protein Design. What I cannot create, I do not understand, Science 346, 1455-1456.
December 11, 2014
Dickson, V. K., Pedi, L., and Long, S. B. (2014) Structure and insights into the function of a Ca2+-activated Cl- channel, Nature 516, 213-218.
December 4, 2014
Brohawn, S. G., Campbell, E. B., and MacKinnon, R. (2014) Physical mechanism for gating and mechanosensitivity of the human TRAAK K+ channel, Nature 516, 126-130.
Also highlighted here.
November 28, 2014
Wang, R., Persky, N. S., Yoo, B., Ouerfelli, O., Smogorzewska, A., Elledge, S. J., and Pavletich, N. P. (2014) DNA repair. Mechanism of DNA interstrand cross-link processing by repair nuclease FAN1, Science 346, 1127-1130.
November 20, 2014
Xu, Y., Tao, Y., Cheung, L. S., Fan, C., Chen, L.-Q., Xu, S., Perry, K., Frommer, W. B., and Feng, L. (2014) Structures of bacterial homologues of SWEET transporters in two distinct conformations, Nature 515, 448-452.
Also featured in Argonne Today.
October 31, 2014
Schirle, N. T., Sheu-Gruttadauria, J., and MacRae, I. J. (2014) Structural basis for microRNA targeting, Science 346, 608-613.
Featured in a comment: Patel, D. J. (2014) RNA. Complete pairing not needed, Science 346, 542-543.
October 30, 2014
McGinty, R. K., Henrici, R. C., and Tan, S. (2014) Crystal structure of the PRC1 ubiquitylation module bound to the nucleosome, Nature 514, 591-596.
Also highlighted in News & Views: Muller, J., and Muller, C. W. (2014) Structural biology: Enzyme-chromatin complex visualized, Nature 514, 572-573.
October 17, 2014
Yang, T., Liu, Q., Kloss, B., Bruni, R., Kalathur, R. C., Guo, Y., Kloppmann, E., Rost, B., Colecraft, H. M., and Hendrickson, W. A. (2014) Structure and selectivity in bestrophin ion channels, Science 346, 355-359.
October 9, 2014
Robart, A. R., Chan, R. T., Peters, J. K., Rajashankar, K. R., and Toor, N. (2014) Crystal structure of a eukaryotic group II intron lariat, Nature 514, 193-197.
Also featured in Nature News & Views, Batey, R. T. (2014) Structural biology: Lariat lessons, Nature 514, 173-174. PubMed
August 29, 2014
Yelshanskaya, M. V., Li, M., and Sobolevsky, A. I. (2014) Structure of an agonist-bound ionotropic glutamate receptor, Science 345, 1070-1074.
August 21, 2014
Althoff, T., Hibbs, R. E., Banerjee, S., and Gouaux, E. (2014) X-ray structures of GluCl in apo states reveal a gating mechanism of Cys-loop receptors, Nature 512, 333-337.
August 14, 2014
Dürr, Katharina L., Chen, L., Stein, Richard A., De Zorzi, R., Folea, I. M., Walz, T., McHaourab, Hassane S., and Gouaux, E. (2014) Structure and Dynamics of AMPA Receptor GluA2 in Resting, Pre-Open, and Desensitized States, Cell 158, 778-792.
August 8, 2014
Gagnon, M. G., Lin, J., Bulkley, D., and Steitz, T. A. (2014) Crystal structure of elongation factor 4 bound to a clockwise ratcheted ribosome, Science 345, 684-687.
July 24, 2014
Wasmuth, E. V., Januszyk, K., and Lima, C. D. (2014) Structure of an Rrp6-RNA exosome complex bound to poly(A) RNA, Nature 511, 435-439.
July 3, 2014
Clayton, G. M., Wang, Y., Crawford, F., Novikov, A., Wimberly, B. T., Kieft, J. S., Falta, M. T., Bowerman, N. A., Marrack, P., Fontenot, A. P., Dai, S., and Kappler, J. W. (2014) Structural basis of chronic beryllium disease: linking allergic hypersensitivity and autoimmunity, Cell 158, 132-142.
June 26, 2014
Schauder, C. M., Wu, X., Saheki, Y., Narayanaswamy, P., Torta, F., Wenk, M. R., De Camilli, P., and Reinisch, K. M. (2014) Structure of a lipid-bound extended synaptotagmin indicates a role in lipid transfer, Nature 510, 552-555.
June 20, 2014
Lee, K., Zhong, X., Gu, S., Kruel, A. M., Dorner, M. B., Perry, K., Rummel, A., Dong, M., and Jin, R. (2014) Molecular basis for disruption of E-cadherin adhesion by botulinum neurotoxin A complex, Science 344, 1405-1410.
June 19th, 2014
Sasaki, E., Zhang, X., Sun, H. G., Lu, M. Y., Liu, T. L., Ou, A., Li, J. Y., Chen, Y. H., Ealick, S. E., and Liu, H. W. (2014) Co-opting sulphur-carrier proteins from primary metabolic pathways for 2-thiosugar biosynthesis, Nature 510, 427-431.
Scott, Daniel C., Sviderskiy, Vladislav O., Monda, Julie K., Lydeard, John R., Cho, Shein E., Harper, J. W., and Schulman, Brenda A. (2014) Structure of a RING E3 Trapped in Action Reveals Ligation Mechanism for the Ubiquitin-like Protein NEDD8, Cell 157, 1671-1684
June 13, 2014
Xu, K., Wu, Z., Renier, N., Antipenko, A., Tzvetkova-Robev, D., Xu, Y., Minchenko, M., Nardi-Dei, V., Rajashankar, K. R., Himanen, J., Tessier-Lavigne, M., and Nikolov, D. B. (2014) Structures of netrin-1 bound to two receptors provide insight into its axon guidance mechanism, Science 344, 1275-1279.
June 5, 2014
King, N. P., Bale, J. B., Sheffler, W., McNamara, D. E., Gonen, S., Gonen, T., Yeates, T. O., and Baker, D. (2014) Accurate design of co-assembling multi-component protein nanomaterials, Nature 510, 103-108.
May 22, 2014
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