Primary funding for this project comes from the National Institute of General Medical Sciences (NIGMS) , a division of the National Institutes of Health (NIH). Additional financial support for NE-CAT comes from the member institutions.
National Institutes of Health
Status of NE-CAT Sector 24 Activities
The accelerator was shut down for scheduled preventative maintenance for nearly the entire month of May. These long accelerator shutdown periods, which occur three times a year, are used by the NE- CAT staff to perform their own preventative maintenance on the beamlines and to install and test new capabilities for its users.
The major emphasis during this shutdown period was to develop a new software interface between the Console beamline control system and the MD2 microdiffractometer control system. When delivered, the MD2 was supplied with its own control system and Maatel had also provided an interface to enable the MD2 to communicate with an external control system. However in usage, our users would periodically experience a hang up requiring rebooting of the system due to problems with the supplied interface. Also the communications between the Console control system and the MD2 software through the supplied interface were very slow, appreciably slowing down the automated sample placement robot operation as well as the rate at which data frames could be taken. To alleviate this problem, a new interface software system was developed and installed during the shutdown period. Following resumption of APS accelerator operations on May 27, our staff thoroughly tested the new interface before arrival of the first users. The new interface was found to be highly reliable; totally eliminating the hang up problems previously encountered and permitted must faster operations. The complete cycle of pin dismount and new pin remount on the goniometer using the sample placement robot was decreased from 2-1/2 minutes to 1-1/4 minutes. Also, the rate at which data frames could be taken was increased by a factor of two. Based upon the successful testing of the new interface, this new control interface has now been installed on both beamlines for routine operations.
Several other new features have been added. A triple aperture assembly has been purchased from ACCEL/Maatel. This assembly contains three different sized apertures. From the control area users can now automatically select which of the three aperture sizes they wish to use- avoiding the need to go in and out of the experimental hutch to manually change apertures. A MD2 Kappa attachment for the MD2 is now available for routine use by our users on the 24-ID-C beamline. To facilitate use of the Kappa, we have made available to the users the STAC (Strategy for Aligned Crystals) software. STAC allows the user to automatically determine the correct orientation of the crystal and provides an optimum data acquisition strategy to be used.
July 23, 2015
Lin, D. Y., Huang, S., and Chen, J. (2015) Crystal structures of a polypeptide processing and secretion transporter, Nature 523, 425-430.
June 5, 2015
Wong, Y. L., Anzola, J. V., Davis, R. L., Yoon, M., Motamedi, A., Kroll, A., Seo, C. P., Hsia, J. E., Kim, S. K., Mitchell, J. W., Mitchell, B. J., Desai, A., Gahman, T. C., Shiau, A. K., and Oegema, K. (2015) Reversible centriole depletion with an inhibitor of Polo-like kinase 4, Science 348, 1155-1160.
May 21, 2015
Wang, K. H., Penmatsa, A., and Gouaux, E. (2015) Neurotransmitter and psychostimulant recognition by the dopamine transporter, Nature 521, 322-327.
May 21, 2015
Garnham, C. P., Vemu, A., Wilson-Kubalek, E. M., Yu, I., Szyk, A., Lander, G. C., Milligan, R. A., and Roll-Mecak, A. (2015) Multivalent Microtubule Recognition by Tubulin Tyrosine Ligase-like Family Glutamylases, Cell 161, 1112-1123.
May 21, 2015
Schmidt, A. G., Therkelsen, M. D., Stewart, S., Kepler, T. B., Liao, H. X., Moody, M. A., Haynes, B. F., and Harrison, S. C. (2015) Viral Receptor-Binding Site Antibodies with Diverse Germline Origins, Cell 161, 1026–1034.
April 24, 2015
Rosenberg, O. S., Dovala, D., Li, X., Connolly, L., Bendebury, A., Finer-Moore, J., Holton, J., Cheng, Y., Stroud, R. M., and Cox, J. S. (2015) Substrates Control Multimerization and Activation of the Multi-Domain ATPase Motor of Type VII Secretion, Cell 161, 501-512.
April 23, 2015
Diao, J., Liu, R., Rong, Y., Zhao, M., Zhang, J., Lai, Y., Zhou, Q., Wilz, L. M., Li, J., Vivona, S., Pfuetzner, R. A., Brunger, A. T., and Zhong, Q. (2015) ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes, Nature 520, 563-566.
March 5, 2015
Mateja, A., Paduch, M., Chang, H. Y., Szydlowska, A., Kossiakoff, A. A., Hegde, R. S., and Keenan, R. J. (2015) Protein targeting. Structure of the Get3 targeting factor in complex with its membrane protein cargo, Science 347, 1152-1155.
January 15, 2015
Lin, J., Gagnon, M. G., Bulkley, D., and Steitz, T. A. (2015) Conformational Changes of Elongation Factor G on the Ribosome during tRNA Translocation, Cell 160, 219-227.
January 9, 2015
Leung, J. H., Schurig-Briccio, L. A., Yamaguchi, M., Moeller, A., Speir, J. A., Gennis, R. B., and Stout, C. D. (2015) Structural biology. Division of labor in transhydrogenase by alternating proton translocation and hydride transfer, Science 347, 178-181.
January 1, 2015
Li, X., Roberti, R., and Blobel, G. (2015) Structure of an integral membrane sterol reductase from Methylomicrobium alcaliphilum, Nature 517, 104-107.
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