Primary funding for this project comes from the National Institute of General Medical Sciences (NIGMS) , a division of the National Institutes of Health (NIH). Additional financial support for NE-CAT comes from the member institutions.
National Institutes of Health
Status of NE-CAT Sector 24 Activities
July - August 2008
During this period we were presented first with some bad and then good news from APS. The 2008-2 run, was shortened by about three weeks due to an APS budget shortfall, and concluded on August 12. However, hearing that they would receive approximately $7M of supplemental funding for the remainder of FY2008, APS announced that the 2008-3 user operation would not be shortened as originally feared. Unfortunately, the APS operating schedule for users throughout 2009 still remains uncertain and subject to uncertainties of congressional action on DOE Office of Science’s 2009 budget request.
During the operational months in July and August, NE- CAT users of both insertion beamlines reported successful runs. Both beamlines operated reliably with no users losing any significant amount of beam time due to faults with the beamlines. However, APS experienced a number of failures in accelerator operations during this period most of short duration but one which was lengthy. On Saturday, August 16 APS experienced a 14 hour long accelerator fault. However, in spite of the problems experienced with the accelerator on this day, only one user group of NE- CAT’s beamlines, a group from the University of Chicago, was unable to complete its planned experiments.
The following pie charts summarize scheduled usage of NE- CAT’s beamlines during the last run period, 2008-2.
24-ID-C Usage by Group
24-ID-E Usage by Group
Particularly notable, both beamlines were fully booked. General User usage of 24-ID-C continued to rise to 43% (47% when subtracting “Development” time resulting in the percentage of time actually available for scheduling of users) which is very close to our target of 50% for General Users. Usage of 24-ID-E by General Users has risen to 35% (39%), substantially above our target of 25% for the first year of operation of this beamline. For both beamlines, “Development Time” of ~10% was a little higher than normal during this period due to the need to train NE- CAT’s three new hires and to improve the reliability of the automated sample placement robot on 24-ID-C which was placed into user operation during this period.
Automated Sample Placement Robotic System
During this period, eight research groups used the newly operational automated sample placement robotics system on the 24-ID-C beamline, mounting and dismounting some 500 crystals. All reported satisfactory results with only a relatively few problems in mounts and dismounts encountered for which the problems were quickly resolved during this commissioning phase. With this early successful experience and reliability of the robotic system steadily improving, an increasing number of users are requesting time to use the robot for screening of their crystals.
Status of New MD2 Microdiffractometer
We have been informed by ACCEL that our second MD2 microdiffractometer, scheduled for installation on 24-ID-C, will most likely be shipped to us late in September, too late to be installed in time for the 2008-3 run beginning October 2. Therefore, installation of the new microdiffractometer on 24-ID-C is now scheduled for the December-January shutdown, with user operation beginning at the start of the 2009-1 run.
January 31, 2018
Coleman, J. A., and Gouaux, E. (2018) Structural basis for recognition of diverse antidepressants by the human serotonin transporter, Nat Struct Mol Biol. Epub ahead of print.
January 30, 2018
Lee, S., Choi, J., Mohanty, J., Sousa, L. P., Tome, F., Pardon, E., Steyaert, J., Lemmon, M. A., Lax, I., and Schlessinger, J. (2018) Structures of β-klotho reveal a 'zip code'-like mechanism for endocrine FGF signalling, Nature 553, 501-505.
December 13, 2017
Born, D. A., Ulrich, E. C., Ju, K. S., Peck, S. C., van der Donk, W. A., and Drennan, C. L. (2017) Structural basis for methylphosphonate biosynthesis, Science 358, 1336-1339.
Read the article.
November 27, 2017
October 5, 2017
Xiao, Y., Ng, S., Nam, K. H., and Ke, A. (2017) How type II CRISPR-Cas establish immunity through Cas1-Cas2-mediated spacer integration, Nature 550, 137-141.
Read the article.
September 21, 2017
Hinshaw, S. M., Makrantoni, V., Harrison, S. C., and Marston, A. L. (2017) The Kinetochore Receptor for the Cohesin Loading Complex, Cell 171, 72-84
August 23, 2017
Zhou, Q., Zhou, P., Wang, A. L., Wu, D., Zhao, M., Sudhof, T. C., and Brunger, A. T. (2017) The primed SNARE-complexin-synaptotagmin complex for neuronal exocytosis, Nature 548, 420-425.
June 9, 2017
Yang, H., and Patel, D. J. (2017) Inhibition Mechanism of an Anti-CRISPR Suppressor AcrIIA4 Targeting SpyCas9, Mol Cell 67, 117-127 e115.
July 20, 2017
Wohlever, M. L., Mateja, A., McGilvray, P. T., Day, K. J., and Keenan, R. J. (2017) Msp1 Is a Membrane Protein Dislocase for Tail-Anchored Proteins, Mol Cell 67, 194-202 e196.
May 26, 2017
Feklistov, A., Bae, B., Hauver, J., Lass-Napiorkowska, A., Kalesse, M., Glaus, F., Altmann, K. H., Heyduk, T., Landick, R., and Darst, S. A. (2017) RNA polymerase motions during promoter melting, Science 356, 863-866.
April 14, 2017
Stanek, K. A., Patterson-West, J., Randolph, P. S., and Mura, C. (2017) Crystal structure and RNA-binding properties of an Hfq homolog from the deep-branching Aquificae: conservation of the lateral RNA-binding mode, Acta Crystallogr D Struct Biol 73, 294-315.
March 29, 2017
Yao, G., Lam, K. H., Perry, K., Weisemann, J., Rummel, A., and Jin, R. (2017) Crystal Structure of the Receptor-Binding Domain of Botulinum Neurotoxin Type HA, Also Known as Type FA or H, Toxins (Basel) 9.
February 17, 2017
Lees, J. A., Messa, M., Sun, E. W., Wheeler, H., Torta, F., Wenk, M. R., De Camilli, P., and Reinisch, K. M. (2017) Lipid transport by TMEM24 at ER-plasma membrane contacts regulates pulsatile insulin secretion, Science 355.
February 13, 2017
Shi, K., Carpenter, M. A., Banerjee, S., Shaban, N. M., Kurahashi, K., Salamango, D. J., McCann, J. L., Starrett, G. J., Duffy, J. V., Demir, O., Amaro, R. E., Harki, D. A., Harris, R. S., and Aihara, H. (2017) Structural basis for targeted DNA cytosine deamination and mutagenesis by APOBEC3A and APOBEC3B, Nat Struct Mol Biol 24, 131-139.
January 31, 2017
Nguyen, L. A., Wang, J., and Steitz, T. A. (2017) Crystal structure of Pistol, a class of self-cleaving ribozyme, Proc Natl Acad Sci U S A.
January 11, 2017
Kuk, A. C., Mashalidis, E. H., and Lee, S. Y. (2016) Crystal structure of the MOP flippase MurJ in an inward-facing conformation, Nat Struct Mol Biol, [epub ahead of print]
December 15, 2017
Yang, H., Gao, P., Rajashankar, K. R., and Patel, D. J. (2016) PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease, Cell 167, 1814-1828 e1812.
December 6, 2016
Bozzi, A. T., Bane, L. B., Weihofen, W. A., Singharoy, A., Guillen, E. R., Ploegh, H. L., Schulten, K., and Gaudet, R. (2016) Crystal Structure and Conformational Change Mechanism of a Bacterial Nramp-Family Divalent Metal Transporter, Structure 24, 2102-2114.
November 20, 2016
Dowling, D. P., Miles, Z. D., Kohrer, C., Maiocco, S. J., Elliott, S. J., Bandarian, V., and Drennan, C. L. (2016) Molecular basis of cobalamin-dependent RNA modification, Nucleic Acids Res 44, 9965-9976.
November 3, 2016
Dimitrova, Yoana N., Jenni, S., Valverde, R., Khin, Y., and Harrison, Stephen C. (2016) Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly, Cell 167, 1014-1027 e1012.
October 21, 2016
Rechkoblit, O., Gupta, Y.K., Malik, R., Rajashankar, K.R., Johnson, R.E., Prakash, L., Prakash, S., Aggarwal, A.K. (2016) Structure and mechanism of human PrimPol, a DNA polymerase with primase activity, Science Advances, 2(10) e1601317
October 20, 2016
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