Primary funding for this project comes from the National Institute of General Medical Sciences(NIGMS) , a division of the National Institutes of Health (NIH). Additional financial support for NE-CAT comes from the member institutions.
National Institutes of Health
Status of NE-CAT Sector 24 Activities
July - August 2008
During this period we were presented first with some bad and then good news from APS. The 2008-2 run, was shortened by about three weeks due to an APS budget shortfall, and concluded on August 12. However, hearing that they would receive approximately $7M of supplemental funding for the remainder of FY2008, APS announced that the 2008-3 user operation would not be shortened as originally feared. Unfortunately, the APS operating schedule for users throughout 2009 still remains uncertain and subject to uncertainties of congressional action on DOE Office of Science’s 2009 budget request.
During the operational months in July and August, NE- CAT users of both insertion beamlines reported successful runs. Both beamlines operated reliably with no users losing any significant amount of beam time due to faults with the beamlines. However, APS experienced a number of failures in accelerator operations during this period most of short duration but one which was lengthy. On Saturday, August 16 APS experienced a 14 hour long accelerator fault. However, in spite of the problems experienced with the accelerator on this day, only one user group of NE- CAT’s beamlines, a group from the University of Chicago, was unable to complete its planned experiments.
The following pie charts summarize scheduled usage of NE- CAT’s beamlines during the last run period, 2008-2.
24-ID-C Usage by Group
24-ID-E Usage by Group
Particularly notable, both beamlines were fully booked. General User usage of 24-ID-C continued to rise to 43% (47% when subtracting “Development” time resulting in the percentage of time actually available for scheduling of users) which is very close to our target of 50% for General Users. Usage of 24-ID-E by General Users has risen to 35% (39%), substantially above our target of 25% for the first year of operation of this beamline. For both beamlines, “Development Time” of ~10% was a little higher than normal during this period due to the need to train NE- CAT’s three new hires and to improve the reliability of the automated sample placement robot on 24-ID-C which was placed into user operation during this period.
Automated Sample Placement Robotic System
During this period, eight research groups used the newly operational automated sample placement robotics system on the 24-ID-C beamline, mounting and dismounting some 500 crystals. All reported satisfactory results with only a relatively few problems in mounts and dismounts encountered for which the problems were quickly resolved during this commissioning phase. With this early successful experience and reliability of the robotic system steadily improving, an increasing number of users are requesting time to use the robot for screening of their crystals.
Status of New MD2 Microdiffractometer
We have been informed by ACCEL that our second MD2 microdiffractometer, scheduled for installation on 24-ID-C, will most likely be shipped to us late in September, too late to be installed in time for the 2008-3 run beginning October 2. Therefore, installation of the new microdiffractometer on 24-ID-C is now scheduled for the December-January shutdown, with user operation beginning at the start of the 2009-1 run.
February 21, 2014
Cheng, W., and Li, W. (2014) Structural insights into ubiquinone biosynthesis in membranes, Science 343, 878-881.
January 23, 2014
Zhou, X., Levin, E. J., Pan, Y., McCoy, J. G., Sharma, R., Kloss, B., Bruni, R., Quick, M., and Zhou, M. (2014) Structural basis of the alternating-access mechanism in a bile acid transporter, Nature 505, 569-573.
December 12, 2013
Geng, Y., Bush, M., Mosyak, L., Wang, F., and Fan, Q. R. (2013) Structural mechanism of ligand activation in human GABAB receptor, Nature 504, 254-259.
November 7, 2013
Penmatsa, A., Wang, K. H., and Gouaux, E. (2013) X-ray structure of dopamine transporter elucidates antidepressant mechanism, Nature 503, 85-90.
Wang, H., Goehring, A., Wang, K. H., Penmatsa, A., Ressler, R., and Gouaux, E. (2013) Structural basis for action by diverse antidepressants on biogenic amine transporters, Nature 503, 141-145. Also highlighted in Chemical and Engineering News
August 30, 2013
Chung, B. C., Zhao, J., Gillespie, R. A., Kwon, D. Y., Guan, Z., Hong, J., Zhou, P., and Lee, S. Y. (2013) Crystal structure of MraY, an essential membrane enzyme for bacterial cell wall synthesis, Science 341, 1012-1016.
August 22, 2013
Simanshu, D. K., Kamlekar, R. K., Wijesinghe, D. S., Zou, X., Zhai, X., Mishra, S. K., Molotkovsky, J. G., Malinina, L., Hinchcliffe, E. H., Chalfant, C. E., Brown, R. E., and Patel, D. J. (2013) Non-vesicular trafficking by a ceramide-1-phosphate transfer protein regulates eicosanoids, Nature 500, 463-467.
August 15, 2013
Zhang, J., and Ferre-D'Amare, A. R. (2013) Co-crystal structure of a T-box riboswitch stem I domain in complex with its cognate tRNA, Nature 500, 363-366.
Also, highlighted in News and Views
August 15, 2013
Lomakin, I. B., and Steitz, T. A. (2013) The initiation of mammalian protein synthesis and mRNA scanning mechanism, Nature 500, 307-311.
June 20, 2013
Rivkin, E., Almeida, S. M., Ceccarelli, D. F., Juang, Y. C., Maclean, T. A., Srikumar, T., Huang, H., Dunham, W. H., Fukumura, R., Xie, G., Gondo, Y., Raught, B., Gingras, A. C., Sicheri, F., and Cordes, S. P. (2013) The linear ubiquitin-specific deubiquitinase gumby regulates angiogenesis, Nature 498, 318-324.
June 20, 2013
Law, J. A., Du, J., Hale, C. J., Feng, S., Krajewski, K., Palanca, A. M. S., Strahl, B. D., Patel, D. J., and Jacobsen, S. E. (2013) Polymerase IV occupancy at RNA-directed DNA methylation sites requires SHH1, Nature 498, 385-389.
May 9, 2013
Yang, H., Rudge, D. G., Koos, J. D., Vaidialingam, B., Yang, H. J., and Pavletich, N. P. (2013) mTOR kinase structure, mechanism and regulation, Nature 497, 217-223.
April 25, 2013
Stinson, Benjamin M., Nager, Andrew R., Glynn, Steven E., Schmitz, Karl R., Baker, Tania A., and Sauer, Robert T. (2013) Nucleotide Binding and Conformational Switching in the Hexameric Ring of a AAA+ Machine, Cell 153, 628-639.
February 14, 2013
Oh, Y. S., Gao, P., Lee, K. W., Ceglia, I., Seo, J. S., Zhang, X., Ahn, J. H., Chait, B. T., Patel, D. J., Kim, Y., and Greengard, P. (2013) SMARCA3, a Chromatin-Remodeling Factor, Is Required for p11-Dependent Antidepressant Action, Cell 152, 831-843.
January 31, 2013
Weixlbaumer, A., Leon, K., Landick, R., and Darst, S. A. (2013) Structural Basis of Transcriptional Pausing in Bacteria, Cell 152, 431-441.
January 10, 2013
Strugatsky, D., McNulty, R., Munson, K., Chen, C. K., Soltis, S. M., Sachs, G., and Luecke, H. (2013) Structure of the proton-gated urea channel from the gastric pathogen Helicobacter pylori, Nature 493, 255–258.
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