Primary funding for this project comes from the National Institute of General Medical Sciences (NIGMS) , a division of the National Institutes of Health (NIH). Additional financial support for NE-CAT comes from the member institutions.
National Institutes of Health
Status of NE-CAT Sector 24 Activities
July - August 2008
During this period we were presented first with some bad and then good news from APS. The 2008-2 run, was shortened by about three weeks due to an APS budget shortfall, and concluded on August 12. However, hearing that they would receive approximately $7M of supplemental funding for the remainder of FY2008, APS announced that the 2008-3 user operation would not be shortened as originally feared. Unfortunately, the APS operating schedule for users throughout 2009 still remains uncertain and subject to uncertainties of congressional action on DOE Office of Science’s 2009 budget request.
During the operational months in July and August, NE- CAT users of both insertion beamlines reported successful runs. Both beamlines operated reliably with no users losing any significant amount of beam time due to faults with the beamlines. However, APS experienced a number of failures in accelerator operations during this period most of short duration but one which was lengthy. On Saturday, August 16 APS experienced a 14 hour long accelerator fault. However, in spite of the problems experienced with the accelerator on this day, only one user group of NE- CAT’s beamlines, a group from the University of Chicago, was unable to complete its planned experiments.
The following pie charts summarize scheduled usage of NE- CAT’s beamlines during the last run period, 2008-2.
24-ID-C Usage by Group
24-ID-E Usage by Group
Particularly notable, both beamlines were fully booked. General User usage of 24-ID-C continued to rise to 43% (47% when subtracting “Development” time resulting in the percentage of time actually available for scheduling of users) which is very close to our target of 50% for General Users. Usage of 24-ID-E by General Users has risen to 35% (39%), substantially above our target of 25% for the first year of operation of this beamline. For both beamlines, “Development Time” of ~10% was a little higher than normal during this period due to the need to train NE- CAT’s three new hires and to improve the reliability of the automated sample placement robot on 24-ID-C which was placed into user operation during this period.
Automated Sample Placement Robotic System
During this period, eight research groups used the newly operational automated sample placement robotics system on the 24-ID-C beamline, mounting and dismounting some 500 crystals. All reported satisfactory results with only a relatively few problems in mounts and dismounts encountered for which the problems were quickly resolved during this commissioning phase. With this early successful experience and reliability of the robotic system steadily improving, an increasing number of users are requesting time to use the robot for screening of their crystals.
Status of New MD2 Microdiffractometer
We have been informed by ACCEL that our second MD2 microdiffractometer, scheduled for installation on 24-ID-C, will most likely be shipped to us late in September, too late to be installed in time for the 2008-3 run beginning October 2. Therefore, installation of the new microdiffractometer on 24-ID-C is now scheduled for the December-January shutdown, with user operation beginning at the start of the 2009-1 run.
September 24, 2015
Rodriguez, J. A., Ivanova, M. I., Sawaya, M. R., Cascio, D., Reyes, F. E., Shi, D., Sangwan, S., Guenther, E. L., Johnson, L. M., Zhang, M., Jiang, L., Arbing, M. A., Nannenga, B. L., Hattne, J., Whitelegge, J., Brewster, A. S., Messerschmidt, M., Boutet, S., Sauter, N. K., Gonen, T., and Eisenberg, D. S. (2015) Structure of the toxic core of α-synuclein from invisible crystals, Nature 525, 486-490.
September 3, 2015
Zhou, Q., Lai, Y., Bacaj, T., Zhao, M., Lyubimov, A. Y., Uervirojnangkoorn, M., Zeldin, O. B., Brewster, A. S., Sauter, N. K., Cohen, A. E., Soltis, S. M., Alonso-Mori, R., Chollet, M., Lemke, H. T., Pfuetzner, R. A., Choi, U. B., Weis, W. I., Diao, J., Sudhof, T. C., and Brunger, A. T. (2015) Architecture of the synaptotagmin-SNARE machinery for neuronal exocytosis, Nature 525, 62-67.
August 13, 2015
Bai, Y., McCoy, J. G., Levin, E. J., Sobrado, P., Rajashankar, K. R., Fox, B. G., and Zhou, M. (2015) X-ray structure of a mammalian stearoyl-CoA desaturase, Nature 524, 252–256.
July 23, 2015
Lin, D. Y., Huang, S., and Chen, J. (2015) Crystal structures of a polypeptide processing and secretion transporter, Nature 523, 425-430.
June 5, 2015
Wong, Y. L., Anzola, J. V., Davis, R. L., Yoon, M., Motamedi, A., Kroll, A., Seo, C. P., Hsia, J. E., Kim, S. K., Mitchell, J. W., Mitchell, B. J., Desai, A., Gahman, T. C., Shiau, A. K., and Oegema, K. (2015) Reversible centriole depletion with an inhibitor of Polo-like kinase 4, Science 348, 1155-1160.
May 21, 2015
Wang, K. H., Penmatsa, A., and Gouaux, E. (2015) Neurotransmitter and psychostimulant recognition by the dopamine transporter, Nature 521, 322-327.
May 21, 2015
Garnham, C. P., Vemu, A., Wilson-Kubalek, E. M., Yu, I., Szyk, A., Lander, G. C., Milligan, R. A., and Roll-Mecak, A. (2015) Multivalent Microtubule Recognition by Tubulin Tyrosine Ligase-like Family Glutamylases, Cell 161, 1112-1123.
May 21, 2015
Schmidt, A. G., Therkelsen, M. D., Stewart, S., Kepler, T. B., Liao, H. X., Moody, M. A., Haynes, B. F., and Harrison, S. C. (2015) Viral Receptor-Binding Site Antibodies with Diverse Germline Origins, Cell 161, 1026–1034.
April 24, 2015
Rosenberg, O. S., Dovala, D., Li, X., Connolly, L., Bendebury, A., Finer-Moore, J., Holton, J., Cheng, Y., Stroud, R. M., and Cox, J. S. (2015) Substrates Control Multimerization and Activation of the Multi-Domain ATPase Motor of Type VII Secretion, Cell 161, 501-512.
April 23, 2015
Diao, J., Liu, R., Rong, Y., Zhao, M., Zhang, J., Lai, Y., Zhou, Q., Wilz, L. M., Li, J., Vivona, S., Pfuetzner, R. A., Brunger, A. T., and Zhong, Q. (2015) ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes, Nature 520, 563-566.
March 5, 2015
Mateja, A., Paduch, M., Chang, H. Y., Szydlowska, A., Kossiakoff, A. A., Hegde, R. S., and Keenan, R. J. (2015) Protein targeting. Structure of the Get3 targeting factor in complex with its membrane protein cargo, Science 347, 1152-1155.
January 15, 2015
Lin, J., Gagnon, M. G., Bulkley, D., and Steitz, T. A. (2015) Conformational Changes of Elongation Factor G on the Ribosome during tRNA Translocation, Cell 160, 219-227.
January 9, 2015
Leung, J. H., Schurig-Briccio, L. A., Yamaguchi, M., Moeller, A., Speir, J. A., Gennis, R. B., and Stout, C. D. (2015) Structural biology. Division of labor in transhydrogenase by alternating proton translocation and hydride transfer, Science 347, 178-181.
January 1, 2015
Li, X., Roberti, R., and Blobel, G. (2015) Structure of an integral membrane sterol reductase from Methylomicrobium alcaliphilum, Nature 517, 104-107.
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