Primary funding for this project comes from the National Institute of General Medical Sciences(NIGMS) , a division of the National Institutes of Health (NIH). Additional financial support for NE-CAT comes from the member institutions.
National Institutes of Health
Status of NE-CAT Sector 24 Activities
July - August 2008
During this period we were presented first with some bad and then good news from APS. The 2008-2 run, was shortened by about three weeks due to an APS budget shortfall, and concluded on August 12. However, hearing that they would receive approximately $7M of supplemental funding for the remainder of FY2008, APS announced that the 2008-3 user operation would not be shortened as originally feared. Unfortunately, the APS operating schedule for users throughout 2009 still remains uncertain and subject to uncertainties of congressional action on DOE Office of Science’s 2009 budget request.
During the operational months in July and August, NE- CAT users of both insertion beamlines reported successful runs. Both beamlines operated reliably with no users losing any significant amount of beam time due to faults with the beamlines. However, APS experienced a number of failures in accelerator operations during this period most of short duration but one which was lengthy. On Saturday, August 16 APS experienced a 14 hour long accelerator fault. However, in spite of the problems experienced with the accelerator on this day, only one user group of NE- CAT’s beamlines, a group from the University of Chicago, was unable to complete its planned experiments.
The following pie charts summarize scheduled usage of NE- CAT’s beamlines during the last run period, 2008-2.
24-ID-C Usage by Group
24-ID-E Usage by Group
Particularly notable, both beamlines were fully booked. General User usage of 24-ID-C continued to rise to 43% (47% when subtracting “Development” time resulting in the percentage of time actually available for scheduling of users) which is very close to our target of 50% for General Users. Usage of 24-ID-E by General Users has risen to 35% (39%), substantially above our target of 25% for the first year of operation of this beamline. For both beamlines, “Development Time” of ~10% was a little higher than normal during this period due to the need to train NE- CAT’s three new hires and to improve the reliability of the automated sample placement robot on 24-ID-C which was placed into user operation during this period.
Automated Sample Placement Robotic System
During this period, eight research groups used the newly operational automated sample placement robotics system on the 24-ID-C beamline, mounting and dismounting some 500 crystals. All reported satisfactory results with only a relatively few problems in mounts and dismounts encountered for which the problems were quickly resolved during this commissioning phase. With this early successful experience and reliability of the robotic system steadily improving, an increasing number of users are requesting time to use the robot for screening of their crystals.
Status of New MD2 Microdiffractometer
We have been informed by ACCEL that our second MD2 microdiffractometer, scheduled for installation on 24-ID-C, will most likely be shipped to us late in September, too late to be installed in time for the 2008-3 run beginning October 2. Therefore, installation of the new microdiffractometer on 24-ID-C is now scheduled for the December-January shutdown, with user operation beginning at the start of the 2009-1 run.
January 15, 2015
Lin, J., Gagnon, M. G., Bulkley, D., and Steitz, T. A. (2015) Conformational Changes of Elongation Factor G on the Ribosome during tRNA Translocation, Cell 160, 219-227.
January 9, 2015
Leung, J. H., Schurig-Briccio, L. A., Yamaguchi, M., Moeller, A., Speir, J. A., Gennis, R. B., and Stout, C. D. (2015) Structural biology. Division of labor in transhydrogenase by alternating proton translocation and hydride transfer, Science 347, 178-181.
January 1, 2015
Li, X., Roberti, R., and Blobel, G. (2015) Structure of an integral membrane sterol reductase from Methylomicrobium alcaliphilum, Nature 517, 104-107.
December 19, 2014
Joh, N. H., Wang, T., Bhate, M. P., Acharya, R., Wu, Y., Grabe, M., Hong, M., Grigoryan, G., and DeGrado, W. F. (2014) De novo design of a transmembrane Zn2+-transporting four-helix bundle, Science 346, 1520-1524.
Highlighted in Lupas, A. N. (2014) Protein Design. What I cannot create, I do not understand, Science 346, 1455-1456.
December 11, 2014
Dickson, V. K., Pedi, L., and Long, S. B. (2014) Structure and insights into the function of a Ca2+-activated Cl- channel, Nature 516, 213-218.
December 4, 2014
Brohawn, S. G., Campbell, E. B., and MacKinnon, R. (2014) Physical mechanism for gating and mechanosensitivity of the human TRAAK K+ channel, Nature 516, 126-130.
Also highlighted here.
November 28, 2014
Wang, R., Persky, N. S., Yoo, B., Ouerfelli, O., Smogorzewska, A., Elledge, S. J., and Pavletich, N. P. (2014) DNA repair. Mechanism of DNA interstrand cross-link processing by repair nuclease FAN1, Science 346, 1127-1130.
November 20, 2014
Xu, Y., Tao, Y., Cheung, L. S., Fan, C., Chen, L.-Q., Xu, S., Perry, K., Frommer, W. B., and Feng, L. (2014) Structures of bacterial homologues of SWEET transporters in two distinct conformations, Nature 515, 448-452.
Also featured in Argonne Today.
October 31, 2014
Schirle, N. T., Sheu-Gruttadauria, J., and MacRae, I. J. (2014) Structural basis for microRNA targeting, Science 346, 608-613.
Featured in a comment: Patel, D. J. (2014) RNA. Complete pairing not needed, Science 346, 542-543.
October 30, 2014
McGinty, R. K., Henrici, R. C., and Tan, S. (2014) Crystal structure of the PRC1 ubiquitylation module bound to the nucleosome, Nature 514, 591-596.
Also highlighted in News & Views: Muller, J., and Muller, C. W. (2014) Structural biology: Enzyme-chromatin complex visualized, Nature 514, 572-573.
October 17, 2014
Yang, T., Liu, Q., Kloss, B., Bruni, R., Kalathur, R. C., Guo, Y., Kloppmann, E., Rost, B., Colecraft, H. M., and Hendrickson, W. A. (2014) Structure and selectivity in bestrophin ion channels, Science 346, 355-359.
October 9, 2014
Robart, A. R., Chan, R. T., Peters, J. K., Rajashankar, K. R., and Toor, N. (2014) Crystal structure of a eukaryotic group II intron lariat, Nature 514, 193-197.
Also featured in Nature News & Views, Batey, R. T. (2014) Structural biology: Lariat lessons, Nature 514, 173-174. PubMed
August 29, 2014
Yelshanskaya, M. V., Li, M., and Sobolevsky, A. I. (2014) Structure of an agonist-bound ionotropic glutamate receptor, Science 345, 1070-1074.
August 21, 2014
Althoff, T., Hibbs, R. E., Banerjee, S., and Gouaux, E. (2014) X-ray structures of GluCl in apo states reveal a gating mechanism of Cys-loop receptors, Nature 512, 333-337.
August 14, 2014
Dürr, Katharina L., Chen, L., Stein, Richard A., De Zorzi, R., Folea, I. M., Walz, T., McHaourab, Hassane S., and Gouaux, E. (2014) Structure and Dynamics of AMPA Receptor GluA2 in Resting, Pre-Open, and Desensitized States, Cell 158, 778-792.
August 8, 2014
Gagnon, M. G., Lin, J., Bulkley, D., and Steitz, T. A. (2014) Crystal structure of elongation factor 4 bound to a clockwise ratcheted ribosome, Science 345, 684-687.
July 24, 2014
Wasmuth, E. V., Januszyk, K., and Lima, C. D. (2014) Structure of an Rrp6-RNA exosome complex bound to poly(A) RNA, Nature 511, 435-439.
July 3, 2014
Clayton, G. M., Wang, Y., Crawford, F., Novikov, A., Wimberly, B. T., Kieft, J. S., Falta, M. T., Bowerman, N. A., Marrack, P., Fontenot, A. P., Dai, S., and Kappler, J. W. (2014) Structural basis of chronic beryllium disease: linking allergic hypersensitivity and autoimmunity, Cell 158, 132-142.
June 26, 2014
Schauder, C. M., Wu, X., Saheki, Y., Narayanaswamy, P., Torta, F., Wenk, M. R., De Camilli, P., and Reinisch, K. M. (2014) Structure of a lipid-bound extended synaptotagmin indicates a role in lipid transfer, Nature 510, 552-555.
June 20, 2014
Lee, K., Zhong, X., Gu, S., Kruel, A. M., Dorner, M. B., Perry, K., Rummel, A., Dong, M., and Jin, R. (2014) Molecular basis for disruption of E-cadherin adhesion by botulinum neurotoxin A complex, Science 344, 1405-1410.
June 19th, 2014
Sasaki, E., Zhang, X., Sun, H. G., Lu, M. Y., Liu, T. L., Ou, A., Li, J. Y., Chen, Y. H., Ealick, S. E., and Liu, H. W. (2014) Co-opting sulphur-carrier proteins from primary metabolic pathways for 2-thiosugar biosynthesis, Nature 510, 427-431.
Scott, Daniel C., Sviderskiy, Vladislav O., Monda, Julie K., Lydeard, John R., Cho, Shein E., Harper, J. W., and Schulman, Brenda A. (2014) Structure of a RING E3 Trapped in Action Reveals Ligation Mechanism for the Ubiquitin-like Protein NEDD8, Cell 157, 1671-1684
June 13, 2014
Xu, K., Wu, Z., Renier, N., Antipenko, A., Tzvetkova-Robev, D., Xu, Y., Minchenko, M., Nardi-Dei, V., Rajashankar, K. R., Himanen, J., Tessier-Lavigne, M., and Nikolov, D. B. (2014) Structures of netrin-1 bound to two receptors provide insight into its axon guidance mechanism, Science 344, 1275-1279.
June 5, 2014
King, N. P., Bale, J. B., Sheffler, W., McNamara, D. E., Gonen, S., Gonen, T., Yeates, T. O., and Baker, D. (2014) Accurate design of co-assembling multi-component protein nanomaterials, Nature 510, 103-108.
May 22, 2014
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