Primary funding for this project comes from the National Institute of General Medical Sciences (NIGMS) , a division of the National Institutes of Health (NIH). Additional financial support for NE-CAT comes from the member institutions.
National Institutes of Health
Status of NE-CAT Sector 24 Activities
July - August 2008
During this period we were presented first with some bad and then good news from APS. The 2008-2 run, was shortened by about three weeks due to an APS budget shortfall, and concluded on August 12. However, hearing that they would receive approximately $7M of supplemental funding for the remainder of FY2008, APS announced that the 2008-3 user operation would not be shortened as originally feared. Unfortunately, the APS operating schedule for users throughout 2009 still remains uncertain and subject to uncertainties of congressional action on DOE Office of Science’s 2009 budget request.
During the operational months in July and August, NE- CAT users of both insertion beamlines reported successful runs. Both beamlines operated reliably with no users losing any significant amount of beam time due to faults with the beamlines. However, APS experienced a number of failures in accelerator operations during this period most of short duration but one which was lengthy. On Saturday, August 16 APS experienced a 14 hour long accelerator fault. However, in spite of the problems experienced with the accelerator on this day, only one user group of NE- CAT’s beamlines, a group from the University of Chicago, was unable to complete its planned experiments.
The following pie charts summarize scheduled usage of NE- CAT’s beamlines during the last run period, 2008-2.
24-ID-C Usage by Group
24-ID-E Usage by Group
Particularly notable, both beamlines were fully booked. General User usage of 24-ID-C continued to rise to 43% (47% when subtracting “Development” time resulting in the percentage of time actually available for scheduling of users) which is very close to our target of 50% for General Users. Usage of 24-ID-E by General Users has risen to 35% (39%), substantially above our target of 25% for the first year of operation of this beamline. For both beamlines, “Development Time” of ~10% was a little higher than normal during this period due to the need to train NE- CAT’s three new hires and to improve the reliability of the automated sample placement robot on 24-ID-C which was placed into user operation during this period.
Automated Sample Placement Robotic System
During this period, eight research groups used the newly operational automated sample placement robotics system on the 24-ID-C beamline, mounting and dismounting some 500 crystals. All reported satisfactory results with only a relatively few problems in mounts and dismounts encountered for which the problems were quickly resolved during this commissioning phase. With this early successful experience and reliability of the robotic system steadily improving, an increasing number of users are requesting time to use the robot for screening of their crystals.
Status of New MD2 Microdiffractometer
We have been informed by ACCEL that our second MD2 microdiffractometer, scheduled for installation on 24-ID-C, will most likely be shipped to us late in September, too late to be installed in time for the 2008-3 run beginning October 2. Therefore, installation of the new microdiffractometer on 24-ID-C is now scheduled for the December-January shutdown, with user operation beginning at the start of the 2009-1 run.
June 21, 2016
Yao, G., Zhang, S., Mahrhold, S., Lam, K. H., Stern, D., Bagramyan, K., Perry, K., Kalkum, M., Rummel, A., Dong, M., and Jin, R. (2016) N-Linked Glycosylation of Sv2 Is Required for Binding and Uptake of Botulinum Neurotoxin A, Nat. Struct. Mol. Biol. [Epub ahead of print].
May 26, 2016
Chung, B. C., Mashalidis, E. H., Tanino, T., Kim, M., Matsuda, A., Hong, J., Ichikawa, S., and Lee, S. Y. (2016) Structural insights into inhibition of lipid I production in bacterial cell wall synthesis, Nature 533, 557-5690.
April 28, 2016
Schmidt, H. R., Zheng, S., Gurpinar, E., Koehl, A., Manglik, A., and Kruse, A. C. (2016) Crystal structure of the human σ1 receptor, Nature 532, 527-530.
April 21, 2016
Coleman, J. A., Green, E. M., and Gouaux, E. (2016) X-ray structures and mechanism of the human serotonin transporter, Nature 532, 334-339.
March 17, 2016
Li, L., Park, E., Ling, J., Ingram, J., Ploegh, H., and Rapoport, T. A. (2016) Crystal structure of a substrate-engaged SecY protein-translocation channel, Nature 531, 395-399.
February 25, 2016
Hayes, R. P., Xiao, Y., Ding, F., van Erp, P. B. G., Rajashankar, K., Bailey, S., Wiedenheft, B., and Ke, A. (2016) Structural basis for promiscuous PAM recognition in type I–E Cascade from E. coli, Nature 530, 499-503. PMID: 26863189.
February 18, 2016
Yin, Z., Shi, K., Banerjee, S., Pandey, K. K., Bera, S., Grandgenett, D. P., and Aihara, H. (2016) Crystal structure of the Rous sarcoma virus intasome, Nature 530, 362-366. PMID: 26887497.
February 5, 2016
Petrou, V. I., Herrera, C. M., Schultz, K. M., Clarke, O. B., Vendome, J., Tomasek, D., Banerjee, S., Rajashankar, K. R., Belcher Dufrisne, M., Kloss, B., Kloppmann, E., Rost, B., Klug, C. S., Trent, M. S., Shapiro, L., and Mancia, F. (2016) Structures of aminoarabinose transferase ArnT suggest a molecular basis for lipid A glycosylation, Science 351, 608-612.
January 1, 2016
Saxton, R. A., Knockenhauer, K. E., Wolfson, R. L., Chantranupong, L., Pacold, M. E., Wang, T., Schwartz, T. U., and Sabatini, D. M. (2016) Structural basis for leucine sensing by the Sestrin2-mTORC1 pathway, Science 351, 53-58.
November 12, 2015
Tao, Y., Cheung, L. S., Li, S., Eom, J.-S., Chen, L.-Q., Xu, Y., Perry, K., Frommer, W. B., and Feng, L. (2015) Structure of a eukaryotic SWEET transporter in a homotrimeric complex, Nature 527, 259-263.
October 30, 2015
Jiang, J., Chan, H., Cash, D. D., Miracco, E. J., Ogorzalek Loo, R. R., Upton, H. E., Cascio, D., O'Brien Johnson, R., Collins, K., Loo, J. A., Zhou, Z. H., and Feigon, J. (2015) Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions, Science 350, aab4070.
October 29, 2015
Wei, J., and Tong, L. (2015) Crystal structure of the 500-kDa yeast acetyl-CoA carboxylase holoenzyme dimer, Nature 526, 723-727.
October 22, 2015
Rubinstein, R., Thu, C. A., Goodman, K. M., Wolcott, H. N., Bahna, F., Mannepalli, S., Ahlsen, G., Chevee, M., Halim, A., Clausen, H., Maniatis, T., Shapiro, L., and Honig, B. (2015) Molecular Logic of Neuronal Self-Recognition through Protocadherin Domain Interactions, Cell 163, 629-642.
Jost, M., Fernandez-Zapata, J., Polanco, M. C., Ortiz-Guerrero, J. M., Chen, P. Y., Kang, G., Padmanabhan, S., Elias-Arnanz, M., and Drennan, C. L. (2015) Structural basis for gene regulation by a B12-dependent photoreceptor, Nature 526, 536–541. PMID: 26416754. 24-ID-C; Pilatus
September 24, 2015
Rodriguez, J. A., Ivanova, M. I., Sawaya, M. R., Cascio, D., Reyes, F. E., Shi, D., Sangwan, S., Guenther, E. L., Johnson, L. M., Zhang, M., Jiang, L., Arbing, M. A., Nannenga, B. L., Hattne, J., Whitelegge, J., Brewster, A. S., Messerschmidt, M., Boutet, S., Sauter, N. K., Gonen, T., and Eisenberg, D. S. (2015) Structure of the toxic core of α-synuclein from invisible crystals, Nature 525, 486-490.
September 3, 2015
Zhou, Q., Lai, Y., Bacaj, T., Zhao, M., Lyubimov, A. Y., Uervirojnangkoorn, M., Zeldin, O. B., Brewster, A. S., Sauter, N. K., Cohen, A. E., Soltis, S. M., Alonso-Mori, R., Chollet, M., Lemke, H. T., Pfuetzner, R. A., Choi, U. B., Weis, W. I., Diao, J., Sudhof, T. C., and Brunger, A. T. (2015) Architecture of the synaptotagmin-SNARE machinery for neuronal exocytosis, Nature 525, 62-67.
August 13, 2015
Bai, Y., McCoy, J. G., Levin, E. J., Sobrado, P., Rajashankar, K. R., Fox, B. G., and Zhou, M. (2015) X-ray structure of a mammalian stearoyl-CoA desaturase, Nature 524, 252–256.
July 23, 2015
Lin, D. Y., Huang, S., and Chen, J. (2015) Crystal structures of a polypeptide processing and secretion transporter, Nature 523, 425-430.
June 19, 2015
Winter, G. E., Buckley, D. L., Paulk, J., Roberts, J. M., Souza, A., Dhe-Paganon, S., and Bradner, J. E. (2015) Phthalimide conjugation as a strategy for in vivo target protein degradation, Science 348, 1376-1381.
June 5, 2015
Wong, Y. L., Anzola, J. V., Davis, R. L., Yoon, M., Motamedi, A., Kroll, A., Seo, C. P., Hsia, J. E., Kim, S. K., Mitchell, J. W., Mitchell, B. J., Desai, A., Gahman, T. C., Shiau, A. K., and Oegema, K. (2015) Reversible centriole depletion with an inhibitor of Polo-like kinase 4, Science 348, 1155-1160.
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