Primary funding for this project comes from the National Institute of General Medical Sciences (NIGMS) , a division of the National Institutes of Health (NIH). Additional financial support for NE-CAT comes from the member institutions.
National Institutes of Health
NE-CAT’s Policy for General Users at the 24ID-C Beam Line
1. General Users are responsible to make sure that they are current in all mandatory APS training requirements. APS training must be completed before coming to the beam line.
2. General Users should follow the mandatory APS dress code (no open-toe shoes, no shorts, no exposed legs).
3. General Users should read in advance the operation manual for the beam line which is located at http://necat.chem.cornell.edu/~igor/24ID-C_manual.pdf.
4. It is user’s responsibility to ensure that all ESAF information have been entered at www.necat.org.
5. All participants from a group must arrive at the start of the time slot for coordinated training at the beam line. The group spokesperson should promptly inform the beam line administrator of any possible delays in arrival. Any beam time cancellation notices should be given at least two weeks before the beam time.
6. The users are solely responsible for timely arrival at the beam line of all shipped materials, e.g., sample shipping Dewars. The users should prepare the return FedEx label and arrange for FedEx pickup of their dry-shipper.
7. Each group must send at least two personnel (of which at least one must have prior experience in using synchrotron beam lines) for the entire time slot. Inexperienced users may not operate the beam line alone. Single person operation, except for short periods of time, will be strongly discouraged. If any damage is caused to the beam line components due to improper handling the cost of repair or procurement will be charged to the users. If it is absolutely necessary for a single person to be operating the beam line, that person should notify in advance the NE-CAT support personnel as well as the floor coordinator. (See NE-CAT policy on “Working Alone” at http://220.127.116.11/~sukumar/work_alone.pdf ).
8. General users should bring to the beam line their own tools necessary for sample manipulation and crystal handling (cryo tongs, crystal wands, forceps, etc). The beam line provides only the cryo-Dewars. and stereo zoom microscope. Any special request for specific tools or use of the chemistry laboratory should be pre-arranged.
9. Hampton-type base/pins with length 18mm are recommended to be used, although a different length can be accommodated (16-21mm). Litho-loops are strongly recommended. Currently, sample mounting is exclusively performed via use of cryo-tongs. Use of mounting arcs is not permitted.Propane frozen samples must be horizontally mounted. Use of Yale mounts should be pre-arranged.
10. Five Linux work stations are available for data processing and structure analysis. Commonly used software such as HKL2000, CCP4 suite, solve/resolve, shelx suite, PHENIX, CNS, etc. are available. General users are not allowed to install or copy any software on the beamline computers. Users can bring their notebook computers to the beam line and connect them to the computer network. Static or DHCP IP numbers will be provided. MAC operating systems are not supported. Use of anti-theft locks for notebooks is highly recommended.
11. General Users must bring to the beam line their own external disk drives to archive their data. Use of USB2/Firewire drives for archiving data is the only way to keep up with the high data flow. Only external hard disks with Linux ext3 format are supported. At least one 300-400GB disk drive is necessary in order to archive one day of data collection.
12. General Users are required to fill out a short “End of Run” summary form before they finish using the beam line. This form is used to summarize their experiments, their experience using the beam line, and providing any suggestions they may have for improvements.
13. General Users are required to cooperate with beam line staff in writing a short summary on their projects that used NE-CAT beam lines for inclusion in NE-CAT’s annual report to our funding agency.
14. If the use of NE-CAT’s beam line results in a publication, the authors must include information in the acknowledgement. Updated acknowledgement requirements can be found -here-.
December 13, 2017
Born, D. A., Ulrich, E. C., Ju, K. S., Peck, S. C., van der Donk, W. A., and Drennan, C. L. (2017) Structural basis for methylphosphonate biosynthesis, Science 358, 1336-1339.
Read the article.
November 27, 2017
October 5, 2017
Xiao, Y., Ng, S., Nam, K. H., and Ke, A. (2017) How type II CRISPR-Cas establish immunity through Cas1-Cas2-mediated spacer integration, Nature 550, 137-141.
Read the article.
September 21, 2017
Hinshaw, S. M., Makrantoni, V., Harrison, S. C., and Marston, A. L. (2017) The Kinetochore Receptor for the Cohesin Loading Complex, Cell 171, 72-84
August 23, 2017
Zhou, Q., Zhou, P., Wang, A. L., Wu, D., Zhao, M., Sudhof, T. C., and Brunger, A. T. (2017) The primed SNARE-complexin-synaptotagmin complex for neuronal exocytosis, Nature 548, 420-425.
June 9, 2017
Yang, H., and Patel, D. J. (2017) Inhibition Mechanism of an Anti-CRISPR Suppressor AcrIIA4 Targeting SpyCas9, Mol Cell 67, 117-127 e115.
July 20, 2017
Wohlever, M. L., Mateja, A., McGilvray, P. T., Day, K. J., and Keenan, R. J. (2017) Msp1 Is a Membrane Protein Dislocase for Tail-Anchored Proteins, Mol Cell 67, 194-202 e196.
May 26, 2017
Feklistov, A., Bae, B., Hauver, J., Lass-Napiorkowska, A., Kalesse, M., Glaus, F., Altmann, K. H., Heyduk, T., Landick, R., and Darst, S. A. (2017) RNA polymerase motions during promoter melting, Science 356, 863-866.
April 14, 2017
Stanek, K. A., Patterson-West, J., Randolph, P. S., and Mura, C. (2017) Crystal structure and RNA-binding properties of an Hfq homolog from the deep-branching Aquificae: conservation of the lateral RNA-binding mode, Acta Crystallogr D Struct Biol 73, 294-315.
March 29, 2017
Yao, G., Lam, K. H., Perry, K., Weisemann, J., Rummel, A., and Jin, R. (2017) Crystal Structure of the Receptor-Binding Domain of Botulinum Neurotoxin Type HA, Also Known as Type FA or H, Toxins (Basel) 9.
February 17, 2017
Lees, J. A., Messa, M., Sun, E. W., Wheeler, H., Torta, F., Wenk, M. R., De Camilli, P., and Reinisch, K. M. (2017) Lipid transport by TMEM24 at ER-plasma membrane contacts regulates pulsatile insulin secretion, Science 355.
February 13, 2017
Shi, K., Carpenter, M. A., Banerjee, S., Shaban, N. M., Kurahashi, K., Salamango, D. J., McCann, J. L., Starrett, G. J., Duffy, J. V., Demir, O., Amaro, R. E., Harki, D. A., Harris, R. S., and Aihara, H. (2017) Structural basis for targeted DNA cytosine deamination and mutagenesis by APOBEC3A and APOBEC3B, Nat Struct Mol Biol 24, 131-139.
January 31, 2017
Nguyen, L. A., Wang, J., and Steitz, T. A. (2017) Crystal structure of Pistol, a class of self-cleaving ribozyme, Proc Natl Acad Sci U S A.
January 11, 2017
Kuk, A. C., Mashalidis, E. H., and Lee, S. Y. (2016) Crystal structure of the MOP flippase MurJ in an inward-facing conformation, Nat Struct Mol Biol, [epub ahead of print]
December 15, 2017
Yang, H., Gao, P., Rajashankar, K. R., and Patel, D. J. (2016) PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease, Cell 167, 1814-1828 e1812.
December 6, 2016
Bozzi, A. T., Bane, L. B., Weihofen, W. A., Singharoy, A., Guillen, E. R., Ploegh, H. L., Schulten, K., and Gaudet, R. (2016) Crystal Structure and Conformational Change Mechanism of a Bacterial Nramp-Family Divalent Metal Transporter, Structure 24, 2102-2114.
November 20, 2016
Dowling, D. P., Miles, Z. D., Kohrer, C., Maiocco, S. J., Elliott, S. J., Bandarian, V., and Drennan, C. L. (2016) Molecular basis of cobalamin-dependent RNA modification, Nucleic Acids Res 44, 9965-9976.
November 3, 2016
Dimitrova, Yoana N., Jenni, S., Valverde, R., Khin, Y., and Harrison, Stephen C. (2016) Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly, Cell 167, 1014-1027 e1012.
October 21, 2016
Rechkoblit, O., Gupta, Y.K., Malik, R., Rajashankar, K.R., Johnson, R.E., Prakash, L., Prakash, S., Aggarwal, A.K. (2016) Structure and mechanism of human PrimPol, a DNA polymerase with primase activity, Science Advances, 2(10) e1601317
October 20, 2016
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