Crystal structure of the RNA demethylase ALKBH5 from zebrafish.
Publication Type:Journal Article
Source:FEBS Lett, Volume 588, Issue 6, p.892-8 (2014)
Keywords:AlkB Homolog 5, RNA Demethylase, Amino Acid Sequence, Animals, Catalytic Domain, Coordination Complexes, Crystallography, X-Ray, Dioxygenases, Hydrogen Bonding, Manganese, Membrane Proteins, Models, Molecular, Molecular Sequence Data, Protein Binding, Protein Structure, Secondary, Sequence Homology, Amino Acid, Zebrafish, Zebrafish Proteins
<p>ALKBH5, a member of AlkB family proteins, has been reported as a mammalian N(6)-methyladenosine (m(6)A) RNA demethylase. Here we report the crystal structure of zebrafish ALKBH5 (fALKBH5) with the resolution of 1.65Å. Structural superimposition shows that fALKBH5 is comprised of a conserved jelly-roll motif. However, it possesses a loop that interferes potential binding of a duplex nucleic acid substrate, suggesting an important role in substrate selection. In addition, several active site residues are different between the two known m(6)A RNA demethylases, ALKBH5 and FTO, which may result in their slightly different pathways of m(6)A demethylation. </p>