Data collection for crystallographic structure determination.
Publication Type:Journal Article
Source:Methods Mol Biol, Volume 1140, p.211-37 (2014)
Keywords:Crystallography, X-Ray, Molecular Biology, Protein Conformation, Statistics as Topic, X-Ray Diffraction
<p>Diffraction data measurement is the final experimental step of crystal structure analysis; all subsequent stages are computational. Good-quality data, optimized for a particular application, make the structure solution and refinement easier and enhance the accuracy of the final models. This chapter describes the principles of the rotation method of data collection and discusses various scenarios that are useful for different types of applications, such as anomalous phasing, molecular replacement, ligand identification, etc. Some typical problems encountered in practice are also discussed.</p>