Mispairs with Watson-Crick base-pair geometry observed in ternary complexes of an RB69 DNA polymerase variant.
Publication Type:Journal Article
Source:Protein Sci, Volume 23, Issue 4, p.508-13 (2014)
Keywords:Base Pair Mismatch, Base Pairing, DNA-Directed DNA Polymerase, Genetic Variation, Models, Molecular, Molecular Conformation, Mutation, Protein Engineering, Viral Proteins
<p>Recent structures of DNA polymerase complexes with dGMPCPP/dT and dCTP/dA mispairs at the insertion site have shown that they adopt Watson-Crick geometry in the presence of Mn(2+) indicating that the tautomeric or ionization state of the base has changed. To see whether the tautomeric or ionization state of base-pair could be affected by its microenvironment, we determined 10 structures of an RB69 DNA polymerase quadruple mutant with dG/dT or dT/dG mispairs at position n-1 to n-5 of the Primer/Template duplex. Different shapes of the mispairs, including Watson-Crick geometry, have been observed, strongly suggesting that the local environment of base-pairs plays an important role in their tautomeric or ionization states.</p>