Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis.

Publication Type:

Journal Article

Authors:

Jenkins, Amy L; Zhang, Yang; Ealick, Steven E; Begley, Tadhg P

Source:

Bioorg Chem, Volume 36, Issue 1, p.29-32 (2008)

Keywords:

Bacillus subtilis, Binding Sites, Catalysis, Hydrolases, Hydrolysis, Models, Molecular, Molecular Structure, Mutagenesis, Site-Directed, Structure-Activity Relationship, Thiamine

Abstract:

<p>TenA catalyzes the hydrolysis of 4-amino-5-aminomethyl-2-methylpyrimidine and participates in the salvage of base-degraded thiamin. Here, we describe mutagenesis of the active site of TenA guided by structures of the enzyme complexed to a substrate analog and to the product. Catalytic roles for each of the active site residues are identified and a mechanism for the reaction is described.</p>