The crystal structure of the human smacovirus 1 Rep domain.

Publication Type:

Journal Article

Authors:

Limón, Lidia K; Shi, Ke; Dao, Amy; Rugloski, Jacob; Tompkins, Kassidy J; Aihara, Hideki; Gordon, Wendy R; Evans, Robert L

Source:

Acta Crystallogr F Struct Biol Commun, Volume 79, Issue Pt 12, p.295-300 (2023)

Keywords:

Crystallography, X-Ray, DNA, Single-Stranded, DNA, Viral, Endonucleases, Humans

Abstract:

<p>Replication initiator proteins (Reps) from the HUH endonuclease family process specific single-stranded DNA sequences to initiate rolling-circle replication in viruses. Here, the first crystal structure of the apo state of a Rep domain from the smacovirus family is reported. The structure of the human smacovirus 1 Rep domain was obtained at 1.33 Å resolution and represents an expansion of the HUH endonuclease superfamily, allowing greater diversity in bioconjugation-tag applications.</p>

PDB: 
8FR5
Detector: 
EIGER2
Beamline: 
24-ID-C