Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein.

Publication Type:

Journal Article

Authors:

Jorda, J; Leibly, D J; Thompson, M C; Yeates, T O

Source:

Chem Commun (Camb), Volume 52, Issue 28, p.5041-4 (2016)

Keywords:

Bacterial Proteins, Molecular Structure, Nanostructures, Nanotechnology, Protein Engineering, Salmonella typhimurium

Abstract:

<p>We report the crystal structure of a novel 60-subunit dodecahedral cage that results from self-assembly of a re-engineered version of a natural protein (PduA) from the Pdu microcompartment shell. Biophysical data illustrate the dependence of assembly on solution conditions, opening up new applications in microcompartment studies and nanotechnology.</p>

PDB: 
5HPN
Detector: 
PILATUS
Beamline: 
24-ID-C