Expression of an 8R-Lipoxygenase From the Coral Plexaura homomalla.

Publication Type:

Journal Article

Authors:

Gilbert, Nathaniel C; Neau, David B; Newcomer, Marcia E

Source:

Methods Enzymol, Volume 605, p.33-49 (2018)

Abstract:

<p>Methods are presented for the use of the coral 8R-lipoxygenase from the Caribbean sea whip coral Plexaura homomalla as a model enzyme for structural studies of animal lipoxygenases. The 8R-lipoxygenase is remarkably stable and can be stored at 4&deg;C for 3 months with virtually no loss of activity. In addition, an engineered &quot;pseudo wild-type&quot; enzyme is soluble in the absence of detergents, which helps facilitate the preparation of enzyme:substrate complexes.</p>