Low Resolution Model Building in EM and X-ray Crystallography Mini-Symposium

SBGrid and NE-CAT are pleased to host a mini-symposium on Low-Resolution Model Building in EM and X-ray Crystallography March 21-22 2019 at Harvard Medical School in Boston. Agenda below. 
For information on lodging and location, please see the event website.

Mini-symposium on Low-resolution model building in EM and X-ray crystallography

March 21-22, 2019, Boston MA


WORKSHOP IS FULL. Please email events@sbgrid.org with questions.

Frank Murphy and Piotr Sliz 

Registration Fee: $100 (includes breakfast, lunch, and happy hour reception)

Preliminary Program

Thursday, March 21:
Room 250, Tosteson Medical Education Center (TMEC) 260 Longwood Ave

  • 8:20- 8:50am:  Breakfast
  • 8:50- 8:55am:  Welcome Remarks (Stephen Blacklow)
  • 8:55- 9:00am:  Opening Remarks (Frank Murphy & Piotr Sliz)
  • 9:00- 9:45am:  Opening Keynote: The unique challenges of modeling complexes purified from native sources (Alan Brown)
  • 9:45- 10:15am:  X-ray Crystallography to Cryo-Electron Microscopy: Software and Data Infrastructure for Structural Biology (Piotr Sliz)
  • 10:15-10:30am:  Coffee Break
  • 10:30-11:00am: Rapid evaluation of EM datasets (Shaun Rawson)
  • 11:00-11:45am:  Structure determination of the mammalian ryanodine receptor by cryoEM (Oliver Clarke)
  • 11:00-11:45am:  Homology-based low-resolution refinement and model rebuilding in PDB-REDO (Tassos Perakis)
  • 12:30-1:30pm:  Lunch 
  • 1:30-2:00pm:  Structure Determination of the DASH/Dam1 Kinetochore Complex by CryoEM (Simon Jeni)
  • 2:00-2:30:   Analyzing Conformational Dynamics of Flexible Domains with Single-Particle Cryo-EM (Ben Orlando)
  • 2:30-2:45:   Break
  • 2:45-3:30pm:   Flexible Fitting into electron densities of heterogenous local resolution - the pros and cones of MD-based refinements (Abhishek Singharoy)
  • 3:30-4:00pm:   Low-resolution lessons from NE-CAT beamline (Frank Murphy)
  • 4:00-4:30pm:   High-performance, Low-Resolution compute infrastructure. Clusters, GPU and Clouds (Jason Key)
  • 4:30-5:00pm:   Structural Determination of Post-translational Translocation Machinery from Yeast (Xudong Wu)
  • 5:00-6:00pm:   Special Keynote: X-ray crystallography and cryo-EM: synergies and complementarities (Stephen Harrison)
  • 6:00-900pm:   Reception and Poster Session - TMEC Atrium

Friday, March 22: 
Room 227, Tosteson Medical Education Center (TMEC) 260 Longwood Ave

  • 8:30-9:00am:   Breakfast 
  • 9:00-10:00am:   Low-resolution model-building (Tom Terwilliger)
  • 10:00-10:45am:   X-ray and Cryo-EM refinement (Pavel Afonine)
  • 10:45-11:15am:   Coffee 
  • 11:15-12:00pm:   Validation for Crystallography and Cryo-EM (Pavel Afonine)
  • 12:00-1:00pm:   Facilitating Rosetta workflows with Cyrus (Brandon Frenz)
  • 1:00-2:00pm:   Lunch 
  • 2:10-2:50pm:   (3) 10-min Lightning Talk from Poster Award Recipients
  • 3:00-5:00:   Open Discussion with Developers, SBGrid, and NE-CAT & EM Facility Tours 
  • 5:00pm:   BCMP Happy Hour/Departure 

See the event website for information on location, lodging, and other logistical details.